Swab device and method for collecting and analyzing a sample

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435292, 435295, 436807, 436810, 436518, 604 1, 604 2, G01N 3353

Patent

active

052504123

ABSTRACT:
A swab device, method, and kit for collecting and analyzing a sample for an analyte (antigens or antibodies) on the swab. The device includes a narrow rod or stick-like support in combination with an assay medium which is positioned adjacent to the support. The assay medium is extended longitudinally down the support a length sufficient to permit an immunoassay to be carried out within it. The assay medium and the support may include a capillary medium. A collection medium may also be attached to the capillary medium to enhance sample collection. The method includes collecting a sample suspected of containing the analyte in a sample collection area at the end of the swab. A liquid containing a labelled component is applied to the swab and allowed to immunochemically react with the sample. If the analyte is present, an immunocomplex is formed and retained on the swab. A wash solution is subsequently applied to the swab to effect the separation of the immunocomplex from excess free label. After separation, the presence or quantity of the analyte in the sample is determined by detecting the label bound to the immunocomplex.

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