Surrogate cell-based system and method for assaying the...

Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...

Reexamination Certificate

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C435S005000, C435S006120, C435S007720, C435S007900, C435S007910, C435S008000, C435S021000, C435S023000, C435S069100, C435S456000

Reexamination Certificate

active

10328206

ABSTRACT:
The present invention concerns the development of a cell-based assay system having improved sensitivity to HCV NS3 protease activity when compared to known assays, which is useful for screening test compounds capable of modulating (particularly inhibiting) HCV NS3 protease activity. This system provides a first construct comprising a transactivator domain joined downstream of the NS3–5 domains of HCV under the control of a non-cytopathic viral promoter system. A second construct is also provided that comprises a reporter gene under the control of an operator sensitive to the binding of the transactivator. The NS3–5 domains encodes the NS3 polyprotein which comprises: the NS3 protease, followed by the NS4A co-factor, the NS4B and NS5A proteins (including any derivative, variant or fragment thereof), terminated by the NS5B protein (including any derivative, variant or fragment thereof) sufficient to constitute a NS5A/5B cleavage site. The transactivator, when expressed and released from the polyprotein initiates transcription and expression of the reporter gene that is measurable.

REFERENCES:
patent: 5861267 (1999-01-01), Su
patent: 6280940 (2001-08-01), Potts et al.
patent: WO98/00548 (1998-01-01), None
patent: WO 00/08469 (2000-02-01), None
patent: WO 00/12727 (2000-03-01), None
Hirowatari et al. (Analytical Biochemistry, 1995, vol. 225, pp. 113-120).
Bowie et al (Science, 1990, 257:1306-1310).
Burgess et al (J. of Cell Bio. 111:2129-2138, 1990).
Bork (Genome Research, 2000,10:398-400).
Lohman, V., et al, Replication of Subgenomic Hepatitis C Virus RNAs in a Hepatoma Cell Line, 1999, Science, 285:110-113.
Kim, J.-E., et al, Subcellular localization of hepatitis C viral proteins in mammalian cells, 1999, Arch. Virol. 144: 329-343.
Hirowatari, Y., et al, A Novel Method for Analysis of Viral Proteinase Activity Encoded by Hepatitis C Virus in Cultured Cells, 1995 Analytical Biochem. 225: 113-120.
Overton, H., et al, Recombinant baculovirus-expressed NS3 proteinase of hepatitis C virus shows activity in cell-based and in vitro assays, 1995, J. Gen. Virol. 76: 3009-3019.
Song, O-K, et al, Development of an in vivo Assay System Suitable for Screening Inhibitors of Hepatitis C Viral Protease, Mol. Cells, 1996, 6 (2): 183-189.
Cho, Y-G, et al, Construction of hepatitis C-SIN virus recombinants with replicative dependency on hepatitis C virus serine protease activity, 1997, J. Virol. Methods, 65: pp. 201-207.
Cho, Y-G., et al, In vivo assay for hepatitis C viral serine protease activity using a secreted protein, 02 1998, .f Virol. Methods, 72: 109-115.

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