Surfactant assay

Details Surfactant assay Surfactant assay

1979-11-02

1981-09-29

Marantz, Sidney

Chemistry: physical processes

Physical processes

Crystallization

23230R, 23902, 210658, G01N 3108

Patent

active

042920418

ABSTRACT:
Thin layer chromatography is used to separate various surfactants. The support medium is silica gel on a glass or acrylamide plate. The developing solvent is chloroform/methanol/water. After drying, the surfactants are detected at extremely low levels with iodine vapor, rhodamine 6G in methanol or phosphomolybdic acid in ethanol/ether, or radiolabelled surfactant. A method is given for the separation of surfactants from proteins or glycoproteins or lipoproteins or polysaccharides, or lipopolysaccharides.

REFERENCES:
patent: 4064041 (1977-12-01), Halpaap
patent: 4129560 (1978-12-01), Zoltobrocki
J. R. Bodenmiller et al., Anal. Chem., 44(6), 926 (1972).
K. Hayashi, Anal. Biochem., 67, 503-506 (1975).
M. J. Rosen, "Systematic Analysis of Surface--Active Agents", 2nd. Edition, 60, 61, 65, 66, 68, 71-76, Wiley-Interscience, New York, 1972.
"Thin-Layer Chromatography", E. Stahl, ed., 493, 498, 499, Academic Press, New York, 1965.
S. Goldstein et al., Anal. Biochem., 64, 130-135 (1975).
P. W. Holloway, Anal. Biochem., 53, 301-308 (1973).
K. Simons et al., J. Mol. Biol., 80, 119-133 (1973).
Kedar et al., Israel J. Med. Sci., 9 (3), 268 (1973).

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