Surface plasmon sensor, sensing apparatus and sensing method

Optics: measuring and testing – By dispersed light spectroscopy – With sample excitation

Reexamination Certificate

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C356S417000, C250S458100

Reexamination Certificate

active

07898659

ABSTRACT:
A sensor has a sensing surface, to which a specific substance R to be detected can bind. Further, the sensor has a metal portion, at least a portion of which is exposed at the sensing surface, and in which localized plasmons can be excited. The sensor is used in sensing, in which the substance R to be detected is marked with a fluorescent marker Lu that selectively binds to the substance R to be detected and one of two-photon excitation fluorescence and multi-photon excitation fluorescence of the fluorescent marker Lu is detected. Further, the sensing surface is illuminated with measurement light L1having a wavelength that can excite localized plasmons in the metal portion and that is an absorption wavelength of the fluorescent marker Lu, at which the fluorescent marker Lu emits one of the two-photon excitation fluorescence and the multi-photon excitation fluorescence.

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