Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Enzyme or microbial cell is immobilized on or in an...
Patent
1983-10-05
1986-04-08
Naff, David M.
Chemistry: molecular biology and microbiology
Carrier-bound or immobilized enzyme or microbial cell;...
Enzyme or microbial cell is immobilized on or in an...
204403, 435180, 435181, 435288, C12N 1114, C12N 1108, C12N 1106, C12M 140
Patent
active
045813361
ABSTRACT:
Surface-modified electrodes which may be used in electrochemical cells for production of electrical energy comprise an enzyme immobilized on a support. The support consists of at least a monolayer coating of a carbonaceous pyropolymer possessing recurring units containing at least carbon and hydrogen atoms composited on a high surface area refractory inorganic oxide such that the carbonaceous pyropolymer monolayer coating replicates the surface area and macropore volume of the inorganic oxide. The coated support is then treated by impregnation with a water-soluble polyamine followed by contact with a solution of a molar excess of a bifunctional monomeric material to form a copolymer which provides pendant bonding sites. The copolymer is entrapped and adsorbed in the pores of the support material to provide a permanent attachment thereto. The treated support is then contacted with an excess of an enzyme to effect the conjugate attachment of the enzyme to the treated support. The immobilized enzyme will act as a working electrode in the presence of a predetermined substrate such as glucose to provide electrical energy.
REFERENCES:
patent: 3651386 (1972-03-01), Youtsey et al.
patent: 4229536 (1980-10-01), DeFilippi
patent: 4240889 (1980-12-01), Yoda et al.
patent: 4307195 (1981-12-01), Karasama et al.
patent: 4321123 (1982-03-01), Nakamura et al.
DeFilippi Louis J.
Malloy Thomas P.
McBride Thomas K.
Naff David M.
Nelson Raymond H.
Page II William H.
UOP Inc.
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