Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1994-04-28
1998-02-24
Wityshyn, Michael G.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
435285, C12N 500
Patent
active
057211310
ABSTRACT:
A process for forming spatially oriented neo-vascular capillaries comprising: (a) providing a combination ultra-thin film (UTF) pattern of at least one cell adhesion promoter and at least one cell adhesion inhibitor wherein the cell adhesion promoters have a linewidth of between about 50-490 .mu.m; (b) seeding the combination UTF pattern with endothelial cells at an initial seeding cell density; (c) adding a first medium for incubating the seeded endothelial cells until the endothelial cells are grown to confluency; (d) replacing the first medium with a second medium at confluency; and (e) allowing the endothelial cells to differentiate into spatially oriented neo-vascular capillaries.
REFERENCES:
patent: 4789601 (1988-12-01), Banes
patent: 4832759 (1989-05-01), Curtis et al.
patent: 5017975 (1991-05-01), Ogawa
patent: 5077085 (1991-12-01), Schnur et al.
patent: 5079600 (1992-01-01), Schnur et al.
patent: 5108926 (1992-04-01), Klebe
patent: 5202227 (1993-04-01), Matsuda et al.
patent: 5324591 (1994-06-01), Georger, Jr. et al.
Ingber et al, J. Cell. Biol. 109: 317-330, 1989.
Stenger et al., Coplanar Moleculor Assemblies of Amino-and Perfluorinated kylsilanes: Characterization and Geometric Definition of Mammalian Cell Adhesion & Growth, vol. 114 JACS 8435 3442 (1992).
Kleinfeld et al., Controlled outgrowth of Dissociated Neurous on Patterned Substrates, The Journal of Neuroscience, 8(11): 4098-4120 (Nov. 1988).
Testoff et al., Abstract: Endothelial Cell adhesion and growth on geass and Biodegradable Discs of PGA modified with Self-assembled Monolayers of Aminosilanes, 19th annual Meeting of The Society For Biomaterials, Apr. 28-May 2, 1993.
Testoff et al., Aminoand Perfluorosiland Self-assembled monolayers Promote adhesion and growth of Endothelial Cells: Potential Material Surfaces for Dictating Angiogensis, National Symposium, Orlando, Florida, 15 Nov. 1993.
Amato, I., A NEw Kind of Organic Gardening, Science, vol. 258, p. 1084, 13 Nov. 1992.
Harris, A., Behavior of cultured cells on Substrate of Variable adhesiveness, Experimental Cell Research, vol. 77, pp. 285-297, (1973).
Robinson et al., Morphologic Plasticity and Periodicity: Porcine cerebral Microvascular Cells in Culture, In Vitro. Cell Dev. Biol. 26: 169-180 (Feb. 1990).
Clark et al., Topographical Control of Cell Behaviour, Topography and Cell Behaviour, pp. 439-448 (1986) Development (1986), vol. 99.
Clark et al., Topographical control of Cell Behaviour: II. Multiple Grooved substrate, Development 108: 635-644 (1990).
Torimitsu et al., Selective Growth of Sensory Nerve Fibers on Metal Oxide Pattern in Culture, Developmental Brain Research, 51: 128-131 (1990).
Fields et al., Nerve Regeneration Through Artificial Tubular Implants, Progress in Neurobiology, vol. 33, pp. 87-134, (1989).
Hirono et al., Recognition of Artificial Microstructures by Sensory Nerve Fibers in Culture, Brain Research, vol. 446, pp. 189-194 (1988).
Rohr et al., Patterned Growth of Neonatal Rat Heart Cells in Culture, Circulation Research, vol. 68, No. 1, pp. 114-129 (Jan. 1991).
Ivanova et al., The Use of Phospholipid Film for Shaping Cell Cultures, Nature, vol. 242, pp. 200-201 (Mar. 16, 1973).
Hammarback et al., Neurite Extension Across Regions of Low Cell-Substratum Adhesinty: Implications for the Guidepost Hypothesis of Axonal Pathfinding, Developmental Biology, vol. 117, pp. 655-662 (1986).
Massia et al., Covalent surface Immobilization of Arg-Gly-Asp and Tyr-Ile-Gly-Ser-Arg Containing Peptides to obtain well-defined cell-adhesive substrates, analytical Biochemistry, vol. 187, pp. 292-301 (1990).
Gross et al., Recording of Spontaneous Activity with Photoetched Microelectrode Surfaces from Mouse Spinal Neurons in culture, Journal of Neuroscience Methods, vol. 5, pp. 13-22, (1982).
Israel et al., An array of Microelectrodes to stimulate and record from cardiac cells in culture, AM. J. Physiol., vol. 247 (Heart CIrc. Physiol. 16): H669-H674 (1984).
Regehr, et al., Sealing cultured Invertebrate neurous to embedded Dish Electrodes facilitates long-term stimulation and recording, Journal of Neuroscience Mehtods, vol. 30, pp. 91-106 (1989).
Experimental Cell Research; vol. 103, pp. 436 to 439, (1976).
Science, vol. vol. 246, pp. 245 to 247 (13 Oct. 1989).
Chu Chih-Chang
Georger Jacque
Rudolph Alan S.
Spargo Barry J.
Stenger David A.
Karasek John
McDonnell Thomas
Prats Francisco C.
United States of America as represented by the Secretary of the
Wityshyn Michael G.
LandOfFree
Surface modification of polymers with self-assembled monolayers does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Surface modification of polymers with self-assembled monolayers , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Surface modification of polymers with self-assembled monolayers will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1874622