Surface induced dissociation with reflectron time-of-flight mass

Details Surface induced dissociation with reflectron time-of-flight mass Surface induced dissociation with reflectron time-of-flight mass

1991-08-02

1992-09-01

Berman, Jack I.

Radiant energy

Ionic separation or analysis

Ion beam pulsing means with detector synchronizing means

250281, 250282, H01J 4940

Patent

active

051441276

ABSTRACT:
Surface induce dissociation (SID) in a reflectron tandem time-of-flight mass spectrometer is demonstrated using a movable "in-line" SID surface in the reflectron lens. For collisions under 100 eV, SID spectra are measured with a resolution of .about.65 (FWHM) with dissociation efficiencies of 7-15% obtained for most small organic ions. For larger peptide ions (m/z>1200) formed by laser desorption, efficiencies as high as 30-50% are obtained. Surface collisions of polycyclic aromatic hydrocarbon ions can be made to produce abundant pick-up of large, surface-adsorbed species. Attachment of C.sub.1 H.sub.n -C.sub.6 H.sub.n to naphthalene and phenanthrene ions occurs with collision energies between 40-160 eV. Formation efficiency for these ion-adsorbate attachment reactions can be as high as 0.8%. Surface collisions produce no measureable shift in our flight times nor distortion in peak shapes for these species; this indicates the reaction time on the surface must be less thant 160 ns. Theoretical calculations show that these reactions are direct (<300 fs residence on the surface) and thus proceed by an Eley-Rideal mechanism.

REFERENCES:
patent: 4731532 (1988-03-01), Frey et al.
patent: 4851669 (1989-07-01), Aberth
patent: 4988879 (1991-01-01), Zare et al.
patent: 5032722 (1991-07-01), Boesl et al.
Cooks et al., "Collisions of Polyatomic Ions with Surfaces," International Journal of Mass Spectrometry and Ion Processes, 100 (1990) 209-265.
Bier et al., "Tandem Mass Spectrometry Using an In-Line Ion-Surface Collision Device," International Journal of Mass Spectrometry and Ion Processes, 103 (1990) 1-19.
Schey et al., "Ion/Surface Collision Phenomena in an Improved Tandem Time-of-Flight Instrument," International Journal of Mass Spectrometry and Ion Processes, 94 (1989) 144-157.
Bier et al., "A Tandem Quadrupole Mass Spectrometer For the Study of Surface-Induced Dissociation," International Journal of Mass Spectrometry and Ion Processes, 77 (1987) 31-47.
Beavis et al., "Factors Affecting the Ultraviolet Laser Desorption of Proteins," Rapid Communications in Mass Spectrometry, 3, no. 7, (1989) 233-37.
Karas et al., "Ultraviolet-Laser Desorption/Ionization Mass Spectrometry of Femtomolar Amounts of Large Proteins," Biomedical and Environmental Mass Spectrometry, 18 (1989) 841-843.
Ding et al., "Surface.gtoreq.Induced Reactions of Benzene and Pyridine," Proc. 39th ASMS Conf. on Mass Spectrom. & Allied Topics, May, 1991, Nashville, Tenn.

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