Drug – bio-affecting and body treating compositions – Whole live micro-organism – cell – or virus containing – Genetically modified micro-organism – cell – or virus
Reexamination Certificate
2007-08-14
2007-08-14
Vogel, Nancy (Department: 1636)
Drug, bio-affecting and body treating compositions
Whole live micro-organism, cell, or virus containing
Genetically modified micro-organism, cell, or virus
C435S320100, C435S252330, C435S252300
Reexamination Certificate
active
10789164
ABSTRACT:
The present invention relates to a method for expressing each of peptide antibiotics P5 3 and Ana13 35 having amphiphilicity and showing antibacterial, antifungal and anticancer activities 61, 63, 65, 67, 69, 71, on the microbial surface, using a vector containing outer membrane protein genes (pgsBCA) that are derived fromBacillussp. strains and involved in the synthesis of poly-gamma-glutamate. Moreover, the present invention relates to lactic acid-forming bacteria having each of the peptide antibiotics P5 15 and Ana13 43 expressed on their surface, and the use thereof.According to the present invention, the peptide antibiotics can be expressed on the surface of various microorganisms transformed with the surface expression vectors. The inventive method for the surface expression of the peptide antibiotics allows the peptide antibiotics to be mass-produced without a purification process. Thus, the inventive method has very high industrial applicability. Further, the present invention can be applied to other peptide antibiotics besides P5 3 and Ana13 35.
REFERENCES:
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patent: 10-2001-0057837 (2003-03-01), None
patent: WO 03/014360 (2003-02-01), None
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Hahm Kyung-Soo
Hong Seung-Pyo
Jung Chang-Min
Kim Chul-Joong
Lee Dong-Gun
Bioleaders Corporation
LaRiviere Grubman & Payne, LLP
Vogel Nancy
LandOfFree
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