Surface-enhanced raman spectroscopy immunoassay method, composit

Details Surface-enhanced raman spectroscopy immunoassay method, composit Surface-enhanced raman spectroscopy immunoassay method, composit

1994-06-30

1996-10-22

Housel, James C.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

Carrier is inorganic

436164, 436173, 436518, 436536, 436538, 436541, 436801, 436805, G01N 33553

Patent

active

055676286

ABSTRACT:
A method, composition, device, apparatus, and kit for the determination of the presence or amount of an analyte by monitoring an analyte-mediated ligand binding event in a test mixture which contains the analyte to be assayed, a specific binding member, a Raman-active label, and a particulate having a surface capable of inducing a surface-enhanced Raman light scattering. The test mixture is illuminated with a radiation sufficient to cause the Raman-active label in the test mixture to emit a detectable Raman spectrum. The differences in the detected surface-enhanced Raman scattering spectra are dependent upon the amount of the analyte present in the test mixture. Thus, by monitoring these differences, the presence or amount of the analyte are determined.

REFERENCES:
patent: 5266498 (1993-11-01), Tarcha et al.
Rohr et al, "Immunoassay Employing Surface-Enhanced Raman Spectroscopy", Analytical Biochemistry, 182, 1989, pp. 388-398.

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