Chemistry: molecular biology and microbiology – Miscellaneous
Reexamination Certificate
2007-11-20
2007-11-20
Saucier, Sandra E. (Department: 1657)
Chemistry: molecular biology and microbiology
Miscellaneous
Reexamination Certificate
active
10352504
ABSTRACT:
Translationally competent ribosomes are bound to a solid substrate through a specific attachment site. A spatial array of such immobilized ribosomes may be produced on a planar substrate, microbeads, on fiber optics. One or more components of the ribosome complex are preferably labeled, e.g. with a fluorescent dye. Ribosomal RNAs, including mRNA and tRNA; and/or ribosomal proteins may be labeled at specific positions, and arrays of immobilized ribosomes may comprise a panel of different labels and positions of labels. Fluorescence detection can then be used to monitor conformational dynamics and translation rates.
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Blanchard Scott C.
Gonzalez Ruben L.
Puglisi Joseph D.
Bozicevic Field & Francis LLP
Saucier Sandra E.
Sherwood Pamela J.
Singh Satyendra K.
The Board of Trustees of the Leland Stanford Junior University
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