Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Primate cell – per se
Patent
1995-06-07
1998-09-29
Budens, Robert D.
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Primate cell, per se
530350, 530351, 530412, 530413, C12N 508, C12N 522
Patent
active
058145192
ABSTRACT:
The present invention relates to a bioactive molecule, herein referred to as the CD8.sup.+ suppressor molecule, that is produced by the CD8.sup.+ subset of human T-lymphocytes and suppresses type-1 human immunodeficiency virus (HIV-1) replication through inhibition of viral transcription. The invention relates to isolation of clonal CD8.sup.+ cells lines that produce the antiviral activity and the development of an assay system for detection of the antiviral activity. The clonal cell lines and the assay system, described herein, may be utilized to purify, characterize and clone the CD8.sup.+ suppressor molecule. The CD8.sup.+ suppressor molecule may have therapeutic applications for treatment of diseases associated with HIV-1 infection.
REFERENCES:
patent: 5196524 (1993-03-01), Gustafson et al.
Powell, J. D. et al. 1991 Ann. N.Y. Acad. Sci. USA vol. 636 pp. 360-363.
Powell, J. D. et al. 1993 Chem. Exp. Immunol. vol. 91 pp. 473-481.
Markovicz, C. et al. 1992 AIDWS Res. Human Retrovir vol. 8 pp. 1039-1050.
Stein, D.S. et al. 1993. Chemical Infection Diseases vol. 17 pp. 749-771.
Carson, K.L., 1994, Genetic Engineering News vol. 14 No. 9 pp. 1,24,34.
Powell, J.D. et al. 1990, Sym. Nonhum. Private Models AIDS v. 8 p. 43.
Markewicz, C et al, 1991, Int. Conf. AIDS vol. 7 No. 2 p. 74.
Copeland et al, "CD8 T Lymphocytes-Mediated Suppression of HIV-1 LTR-Mediated Transcription Shows No Correlation With Clinical Stage Of Disease or Health Status," Eleventh Int'l Conference on AIDS, Vancouver, Jul. 7-12, 1996, p. 227, Abstract No. Tu.A.395.
Barker et al., "Effects of TH1 and TH2 Cytokines On CN8.sup.+ Cell Response Against Human Immunodeficiency Virus Implication For Long-Term Survival," Proc. Natl. Acad. Sci. USA 92:11135-11139, Nov. 1995.
Kinter et al., "Interlinkin 2 Induces CD8.sup.+ TCell-Mediated Suppression of Human Immunodeficiency Virus Replication in CD4.sup.+ T Cells And This Effect Overrides Its Ability To Stimulate Virus Expression," Proc. Nat'l.Acad.Sci USA 92:10985-10989, Nov. 1995.
Fahey et al., "Status of Immune Based Therapies In HIV Infection and AIDS," Clin. Exp. Immunol. 88:1-5, Apr. 1997.
Fox, J.L., "No Winners Against AIDS," Bio/Technology 12:128, Feb. 1994.
Barre-Sinoussi, F. et al., 1983; Isolation of a T-Lymphotropic Retrovirus from a Patient at Risk for Acquired Immune Deficiency Syndrome (AIDS). Science 220:868-871.
Gallo, R. et al., 1984, Frequent Detection and Isolation of Cytopathic Retroviruses (HTLV-III) from Patients with AIDS and at Risk for AIDS. Science 224:500-503.
Wong-Staal, F. and Gallo, R., 1985; Human T-Lymphotrophic Retroviruses. Nature 317:395-403.
Walker et al., 1986; CD8.sup.+ Lymphocytes Can Control HIV Infection in Vitro by Supressing Virus Replication. Science 234:1563-1566.
Kannagi, M. et al., 1988; Suppression of Simian Immunodeficiency Virus Replication in Vitro By CD8.sup.+ Lymphocytes. Journal of Immunology 140(7): 2237-2242.
Tsubota, H. et al., 1989; A Cytotoxic T Lymphocye Inhibits Acquired Immunodeficiency Syndrome Virus Replication in Peripheral Blood Lymphocytes. J. Exp. Med. 169:1421-1434.
Walker, C. and Levy, J., 1989; A Diffusable Lymphokine Produced by CD8.sup.+ T Lymphocytes Suppresses HIV Replication. Immunology 66:628-630.
Tsubota, H. et al., 1989; CD8.sup.+ CD4.sup.- Lymphocyte Lines Can Harbor the AIDS Virus In Vitro. Journal of Immunology 143(3):858-863.
Cullen, B. and Greene, W., 1989, 1989; Regulatory Pathways Governing HIV Replication. Cell 58:423-426.
Walker, C. et al. 1989; CD8.sup.+ T Lymphocyte Control of HIV Replication in Cultured CD4.sup.+ Cells Varies among Infected Individuals. Cellular Immunology 119:470-475.
Brinchmann, J. et al., 1989; Reliable Isolation of Human Immunodeficiency Virus from Cultures of Naturally Infected CD4.sup.+ T Cells. Journal of Virological Methods 25:293-300.
Brinchmann, J. et al., 1990; CD8.sup.+ T Cells Inhibit HIV Replication in Naturally Infected CD4.sup.+ T Cells, Evidence for a Soluble Inhibitor. J. Immunol. 144:2961-2966.
Kannagi, M. et al., 1990; Interference with Human Immunodeficiency Virus (HIV) Replication by CD8.sup.+ T Cells in Peripheral Blood Leukocytes of Asymptomatic HIV Carriers In Vitro. Journal of Virology 64(7):3399-3406.
Wivott, L. et al., 1990; CD8.sup.+ Lymphocytes Suppress HIV Production by Autologous CD4.sup.+ Cells without Eliminating the Infected from Culture. Cellular Immunology 128:628-634.
Castro, B., 1991; Suppression of Human Immunodeficiency Virus Replication by CD8.sup.+ Cells from Infected and Uninfected Chimpanzees. Cellular Immunology 132:246-255.
Mackewicz, C., et al., 1991; CD*.sup.+ Cell Anti-HIV Activity Correlates with the Clinical State of the Infected Individual. J. Clin. Invest. 87:1462-1466.
Brinchmann, J. et al., 1991; In Vitro Replication of HIV-1 in Naturally Infected CD4.sup.+ T Cells is Inhibited by rIFN.alpha..sub.2 and by a Soluble Factor Secreted by Activated CD8.sup.+ T Cells, But Not By rIFN.sub..beta., rIFN.varies., or Recombinant Tumor Necrosis Factor-.alpha.. Journal of Acquired Immune Deficiency Syndrome 4(5):480-488.
Walker, C. et al., 1991; CD8.sup.8 Cells from HIV-1-Infected Individuals Inhibit Acute Infection by Human and Primate Immunodeficiency Viruses. Cellular Immunology 137:420-428.
Walker, C. et al., 1991; Inhibition of Human Immunodeficiency Virus Replication in Acutely Infected CD$.sup.+ Cells by CD8.sup.+ Cells Involves a Noncytotoxic Mechanism. J. Virology 65(11):5921-5927.
Powell, J. et al., 1990; Sym. Nonhuman Primate Models AIDS 8:43.
Mackewicz, C. et al., 1991; CD8.sup.+ Lymphocytes from HIV-1 Seropositive Individuals Block HIV Replication in CD4.sup.+ Lymphocytes by Inhibiting Viral RNA Synthesis. Int. Conf. AIDS 7: 74.
Powell, J. et al., 1991; Regulatioin of Immune Activation/Retroviral Replication by CD8.sup.+ T Cells. Ann. N.Y. Acad. Sci. USA 636:360-363.
Mackewicz, C. et al., 1992; CD8.sup.+ Cell Anti-HIV Activity: Non-Lytic Suppression of Virus Replication. AIDS Res. Human Retroviruses 8:1039-1050.
Powell, J. et al., 1993; Inhibition of Cellular Activation of Retroviral Replication by CD8.sup.+ T Cells Derived from Non-Human Primates. Clin. Exp. Immunol. 91:473-481.
Stein, D. et al., 1993; Immune-Based Therapeutics: Scientific Rationale and the Promising Approaches to the Treatment of the Human Immunodeficiency Virus-Infected Individual. Clin. Infct. Dis. 17:749-771.
Carson, K. et al., 1994; Companies Remain Hopeful for Vaccines for AIDS Despite Discouraging Results. Gen. Engineering News 14:1,24,34.
Copeland, K. et al., 1996; CD8.sup.+ Lymphocyte-Mediated Suppression of HIV-1 LTR-Mediated Transcription Shows No Correlation with Clinical Stage of Disease or Health Status. XIth Int'l Con. AIDS, Vancouver, Jul. 7-12:12 (Abs. Tu,A,395).
Fahey, J. and Schooley, R. et al., 1992; Status of Immune-Based Therapies in HIV Infection and AIDS. Clin. Exp. Immunol. 88:1-5.
Fox, J., 1994, No Winners Against AIDS. Bio/Technology 12:128.
Barker, E. et al., 1995; Effects of T.sub.H 1 and T.sub.H 2 Cytokines on CD8.sup.+ Cell response Against Human Immunodeficiency Virus: Implications for Long-Term Survival. Proc. Natl. Acad. Sci. USA 92:11135-11139.
Kinter, A. et al., 1995; Interleukin 2 Induces CD8.sup.+ T Cell-Mediated Suppression of Human Immunodeficiency Virus Replication in CD4.sup.+ T Cells and this Effect Overrides its Ability to Stimulate Virus Expression. Proc. Natl. Acad. Sci. USA 92A:10985-10989.
Burgess, R., 1987; Protein Purication, Micro to Macro. ALan R. Liss, New York. pp. 17-26.
Old, R., et al., 1989; Principles of Gene Manipulation, an Introduction to Genetic Engineering. Blackwell Scientific Publications, Oxford. pp. 137-139.
Anderson et al., 1990; A Monoclonal Antibody Reactive with a 15-kDa Cytoplasmic Granule-Associated Protein Defines Subpopulation of CD8.sup.+ T Lymphocytes. J. Immunol. 144:574-582.
Pantaleo et al., 1992; Defective Clonogenic Potential of CD8.sup.+ T Lymphocytes in Patients with AIDS. Expansion In Vivo Nonclonogenic CD3.sup.+ CD8.sup.+ DR.sup.+ CD25-T Cell Population. J. Immunol. 144:1702.
Chen et al., 1993; CD8.sup.+ T Lymphocyte-Mediated Inhibition of HIV-1 Long Termi
Bolognesi Dani P.
Chen Chin-Ho
Greenberg Michael
Lacey Simon F.
Weinhold Kent
Budens Robert D.
Duke University Mdeical Center
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