Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1993-03-29
1997-05-06
Budens, Robert D.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 724, 435 8, 435 29, 435974, C12Q 102, C12Q 170
Patent
active
056270232
ABSTRACT:
The present invention relates to a bioactive molecule, herein referred to as the CD8 suppressor molecule, that is produced by the CD8 subset of human T-lymphocytes and suppresses type-1 human immunodeficiency virus (HIV-1) replication through inhibition of vital transcription. The invention relates to isolation of clonal CD8 cells lines that produce the antiviral activity and the development of an assay system for detection of the antiviral activity. The clonal cell lines and the assay system, described herein, may be utilized to purify, characterize and clone the CD8 suppressor molecule. The CD8 suppressor molecule may have therapeutic applications for treatment of diseases associated with HIV-1 infection.
REFERENCES:
Barre-Sinoussi, F. et al., 1983; Isolation of a T-Lymphotropic Retrovirus from a Patient at Risk for Acquired Immune Deficiency Syndrome (AIDS). Science 220:868-871.
Gallo, R. et al., 1984; Frequent Detection and Isolation of Cytopathic Retroviruses (HTLV-III) from Patients with AIDS and at Risk for AIDS. Science 224:500-503.
Wong-Staal, F. and Gallo, R., 1985; Human T-Lymphotropic Retroviruses. Nature 317:395-403.
Walker et al., 1986; CD8.sup.+ Lymphocytes Can Control HIV Infection in Vitro by Suppressing Virus Replication. Science 234: 1563-1566.
Kannagi, M. et al., 1988; Suppression of Simian Immunodeficiency Virus Replication in Vitro By CD8+ Lymphocytes. Journal of Immunology 140(7): 2237-2242.
Tsubota, H. et al., 1989; A Cytotoxic T Lymphocyte Inhibits Acquired Immunodeficiency Syndrome Virus Replication in Peripheral Blood Lymphocytes. J. Exp. Med. 169:4121-1434.
Walker, C. and Levy, J., 1989; A Diffusible Lymphokine Produced by CD8.sup.+ T Lymphocytes Suppresses HIV Replication. Immunology 66:628-630.
Tsubota, H. et al., 1989; CD8.sup.+ CD4.sup.- Lymphocyte Lines Can Harbor the AIDS Virus In Vitro. Journal of Immunology 143(3):858-863.
Cullen, B. and Greene, W., 1989; Regulatory Pathways Governing HIV Replication. Cell 58:423-426.
Walker, C. et al., 1989; CD8.sup.+ T Lymphocyte Control of HIV Replication in Cultured CD4.sup.+ Cells Varies among Infected Individuals. Cellular Immunology 119:470-475.
Brinchmann, J. et al., 1989; Reliable Isolation of Human Immunodeficiency Virus from Cultures of Naturally Infected CD4.sup.+ T Cells. Journal of Virological Methods 25:293-300.
Brinchmann, J. et al., 1990; CD8.sup.+ T Cells Inhibit HIV Replication in Naturally Infected CD4.sup.+ T Cells, Evidence for a Soluble Inhibitor. J. Immunol. 144:2961-2966.
Kannagi, M. et al., 1990; Interference with Human Immunodeficiency Virus (HIV) Replication by CD8.sup.+ T Cells in Peripheral Blood Leukocytes of Asymptomatic HIV Carriers In Vitro. Journal of Virology 64(7):3399-3406.
Wiviott, L. et al., 1990; CD8.sup.+ Lymphocytes Suppress HIV Production by Autologous CD4.sup.+ Cells without Eliminating the Infected Cells from Culture. Cellular Immunology 128:628-634.
Castro, B., 1991; Suppression of Human Immunodeficiency Virus Replication by CD8.sup.+ Cells from Infected and Uninfected Chimpanzees. Cellular Immunology 132:246-255.
Mackewicz, C., et al., 1991; CD8.sup.+ Cell Anti-HIV Activity Correlates with the Clinical State of the Infected Individual. J. Clin. Invest. 87:1462-1466.
Brinchmann, J. et al., 1991; In Vitro Replication of HIV-1 in Naturally Infected CD4.sup.+ T Cells is Inhibited by rIFN.alpha..sub.2 and by a Soluble Factor Secreted by Activated CD8.sup.+ T Cells, But Not By rlFN.sub..beta., rIFN.sub..delta., or Recombinant Tumor Necrosis Factor-.alpha.. Journal of Acquired Immune Deficiency Syndrome 4(5):480-488.
Walker, C. et al., 1991; CD8.sup.+ Cells from HIV-1-Infected Individuals Inhibit Acute Infection by Human and Primate Immunodeficiency Viruses. Cellular Immunology 137:420-428.
Walker, C. et al., 1991; Inhibition of Human Immunodeficiency Virus Replication in Acutely Infected CD4.sup.+ Cells by CD8.sup.+ Cells Involves a Noncytotoxic Mechanism. J. Virology 65(11):5921-5927.
Copeland et al., "CD8 T Lymphocyte-Mediated Suppression of HIV-1 LTR-Mediated Transcription Shows No Correlation With Clinical Stage of Disease or Health Status," Eleventh Int.'l. Conference on AIDS, Vancouver, Jul. 7-12, 1996, p. 227, Abstract No. Tu.A.395.
Powell, J. D. et al. 1991. Ann. N.Y. Acad. Sci. USA vol 636 pp. 360-363.
Powell, J. D. et al. 1993. Clin. Exp. Immunol. vol 91 pp. 473-481.
Mackewicz, C. et al. 1992. AIDS Res. Human Retro Vir. vol 8 pp. 1039-1050.
Stein, D.S. et al. 1993. Clinical Infections Diseases vol 17 pp. 749-771.
Carson, K.L., 1994. Genetic Engineering News vol 14 No 9 pp. 1, 24, 34.
Powell, J.D. et al. 1990. Sym. Non hum. Primate Models AIDS v. 8 p. 43.
Mackewicz, C. et al. 1991. Int. Conf. AIDS vol 7 No. 2 p. 74.
Bolognesi Dani P.
Chen Chin-Ho
Greenberg Michael L.
Weinhold Kent
Budens Robert D.
Duke University
LandOfFree
Suppressor of HIV replication and transcription does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Suppressor of HIV replication and transcription, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Suppressor of HIV replication and transcription will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2131567