Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...
Patent
1995-01-19
1997-11-11
Chereskin, Che S.
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide alters fat, fatty oil, ester-type wax, or...
4351723, 435419, 800250, 800DIG67, A01H 400, C12N 1582, C12N 514
Patent
active
056866496
ABSTRACT:
Disclosed is a novel method of suppressing plant gene expression. The suppression is achieved by transforming a plant with a DNA construct encoding a processing-defective RNA (pd-RNA constructs). A pd-RNA construct comprises a plant active promoter operably linked to a pd-RNA encoding segment (pd-RNA segment), wherein the pd-RNA segment comprises a sequence substantially homologous to the target gene and a defective intron and/or a defective 3' termination and processing sequence (hereinafter 3' processing sequence). The pd-RNA constructs of the present invention are designed to express target-gene-homologous RNA transcripts that are defective for messenger RNA processing. Various types of pd-RNA constructs are disclosed, including those defective for endonucleolytic cleavage or polyadenylation at the 3' end of the pd-RNA transcript and/or intron splicing. A pd-RNA construct of the invention may used to suppress a single, specific target gene or multiple target genes. Further, the suppression effect of a pd-RNA construct can be modulated and controlled through the use of an appropriate promoter.
REFERENCES:
patent: 5034323 (1991-07-01), Jorgensen et al.
patent: 5231020 (1993-07-01), Jorgenson et al.
patent: 5283184 (1994-02-01), Jorgensen et al.
Bird and Ray, "Manipulation of Plant Gene Expression by Antisense RNA," Biotechnology and Genetic Engineering Reviews, vol. 9, (Dec. 1991), pp. 207-227.
Jorgensen, "Altered Gene Expression in Plants due to Trans Interactions Between Homologous Genes," Trends Biotechnol., vol. 8, (Dec. 1990), pp. 340-344.
Hooper, "The Petunia Paradox: Added Copies of Genes Have Puzzling Effects in Plants," The Journal of NIH Research, vol. 3, (Dec. 1991), pp. 49-54.
Mattaj, "Splicing Stories and Poly(A) Tales: An Update on RNA Processing and Transport," Current Opinion in Cell Biology, vol. 2, (1990), pp. 528-538.
Izaurralde and Mattaj, "Transparent of RNA Between Nucleus and Cytoplasm," Seminars In Cell Biology, vol. 3, (1992), pp. 279-288.
de Carvalho et al., "Suppression of .beta.-1,3-glucanase Transgene Expression in Homozygous Plants," EMBO J., pp. 2595-2602.
van der Krol et al., "Flavonoid Genes in Petunia: Addition of a Limited Number of Gene Copies May Lead to a Suppresion of Gene Expression," The Plant Cell, vol. 2, (Apr. 1990), pp. 291-299.
Napoli et al., "Introduction of a Chimeric Chalcone Synthase Gene into Petunia Results in reversible Co-Suppresion of Homologous Genes in Trans," The Plant Cell, vol. 2, (Apr. 1990), pp. 279-289.
van der Krol et al., "Functional Analysis of Petunia Floral Homeotic MADS Box Gene pMADS1," Genes & Development, vol. 7, (1993), pp. 1214-1228.
van der Krol et al., "Antisense Genes in Plants: An Overview," Gene, vol. 72, (1988), pp. 45-50.
Chua Nam-Hai
van der Krol Alexander
Chereskin Che S.
The Rockefeller University
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