Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Reexamination Certificate
2011-04-19
2011-04-19
Wang, Shengjun (Department: 1627)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
C435S006120
Reexamination Certificate
active
07927790
ABSTRACT:
FEN-1 is involved in processing HIV central DNA flap (CDF) and is a target for intervention of HIV-1 replication. Inhibition of HIV-1 CDF removal by expression of D181A FEN-1 mutant suppresses viral DNA replication, further demonstrating that FEN-1 is a target. Methods of identifying and using molecules that inhibit FEN-1 processing HIV-1 CDF and other methods of inhibiting FEN-1 processing are provided. Useful small molecules specifically suppress FEN-1 cleavage of HIV CDF but have little effect on FEN-1's function in RNA-DNA primer removal. Pentamidine inhibits HIV replication by suppressing FEN-1-mediated HIV-CDF processing.
REFERENCES:
patent: 6090543 (2000-07-01), Dahlberg et al.
patent: 6348314 (2002-02-01), Dahlberg et al.
patent: WO 9940227 (1999-08-01), None
Bushman, F. D. (1999) Host proteins in retroviral cDNA integration.Adv. Virus Res. 52:301-317.
Charneau, P. and Clavel, F. (1991) A single-stranded gap in human immunodeficiency virus unintegrated linear DNA defined by a central copy of the polypurine tract.J. Virol. 65:2415-2421.
Charneau, P., et al. (1992) A second origin of DNA plus-strand synthesis is required for optimal human immunodeficiency virus replication.J. Viol. 66:2814-2920.
Charneau, P., et al. (1994) HIV-1 reverse transcription. A termination step at the center of the genome.J. Mol. Biol. 241:651-62.
Chen, H., and Engelman, A. (1998) The barrier-to-autointegration protein is a host factor for HIV type 1 integration.Proc. Natl. Acad. Sci. USA. 95:15270-15274.
Decker, T., and M. L. Lohmann-Matthes. (1988) A quick and simple method for the quantitation of lactate dehydrogenase release in measurements of cellular cytotoxicity and tumor necrosis factor (TNF) activity.J lmmunol. Methods115:61-9.
Doranz, B.J., Bail, S.S. et al., (1999) Use of a gp120 binding assay to dissect the requirements and kinetic of human immunodeficiency virus fusion events.J. Viol. 73:10346-10258.
Dvorin, J. D., Bell, P., Maul, G. G., Yamashita, M., Emerman, M., and Malim, M. H. (2002) Reassessment of the role of integrase and the central DNA flap in human immunodeficiency virus type 1 nuclear import.J. Virol. 76:12087-12096.
Farnet, C. M., and Bushman, F. D. (1997) HIV-1 cDNA integration: requirement of HMG 1(Y) protein for function of preintegration complexes in vitro.Cell88:483-492.
Farnet, C. M., and Haseltine, W. A. (1990) Integration of human immunodeficiency virus type 1 DNA in vitro.Proc. Natl. Acad. Sci. USA 87:4164-4168.
Farnet, C.M. and Haseltine, W.A. (1991) Determination of viral proteins present in the human immunodeficiency virus type 1 preintegration complex.J. Viol. 65:1910-1915.
Faust, E. A. and Triller, H. (2002) Stimulation of human flap endonuclease 1 by human immunodeficiency virus type 1 integrase: Possible role for flap endonuclease 1 in 5'-end processing of human immunodeficiency virus type 1 integration intermediates.J. Biomedical Science9:273-287.
Gaur, M., and A. D. Leavitt (1998). Mutations in the human immunodeficiency virus type 1 integrase D,D(35)E motif do not eliminate provirus formation.J. Virol. 72:4678-85.
Harrington, J.J. and Lieber, M. R. (1994) Functional domains within FEN-1 and RAD2 define a family of structure-specific endonucleases: implications for nucleotide excision repair.Genes & Development8:1344-1355.
Harrington, J.J. and Lieber, M. R. (1994) The characterization of a mammalian DNA structure-specific endonuclease.EMBO J.13(5):1235-1246.
Hiraoka, L.R., et al., (1995) Sequence of Human FEN-1, a Structure-Specific Endoneuclease, and Chromosomal Localization of the Gene (FEN1) in Mouse and Human.Genomics25:220-225.
Ho, D. D., et al. (1991) Conformational epitope on gp120 important in CD4 binding and human immunodeficiency virus type 1 neutralization identified by a human monoclonal antibody.J Virol., 65:489-93.
Hungnes, O., et al. (1992) Mutations in the central polypurine tract of HIV-1 result in delayed replication.Virology190:440-442.
Kao, H.-I., and Bambara, R. A. (2003) The protein components and machanism of eukaryotic Okazaki fragment maturation.Critical Reviews in Biochemistry and Molecular Biology38:433-452.
Kucherlapati, M., et al. (2002) Haploinsufficiency of flap endonuclease (Fen-1) leads to rapid tumor progress.Proc. Natl. Acad. Sci. USA 99:9924-9929.
Lieber, M.R. (1997) The Fen-1 family of structure-specific nucleases in eukaryotic DNA replication, recombination and repair.BioEssays. 19:233-240.
Limón, A., et al. (2002) Wild-type levels of nuclear localization and human immunodeficiency virus type 1 replication in the absence of the central DNA flap.J. Virol. 76:12078-12086.
Miller, M. D., et al. (1995) Human immunodeficiency virus type 1 preintegration complexes containing discontinuous plus strand are competent to integrate in vitro.J. Virol. 69:3938-3944.
Miller, M. D., et al. (1997) Human immunodeficiency virus type 1 preintegration complexes: studies of organization and composition.J. Virol. 71:5382-5390.
Murante, R.S., et al. (1995) Calf 5′ to 3′ exo/endonuclease must slide from a 5′ end of the substrate to perform structure-specific cleavage.J. Biol. Chem. 270:30377-83.
Murray, J.M., et al., (1994) Structural and Functional Conservation of the Human Homolog of theSchizosaccharomyces ponbe rad2gene, Which Is Required for Chromosome Segregation and Recovery from DNA Damgage.Molecular and Cellular Biology14:4878-4886.
Parrish, J., et al. (2003) CRN-1, aCaenorhabditis elegansFEN-1 homologue, cooperates with CPS-6/EndoG to promot apoptotic DNA degradation.EMBO J. 22(13):3451-3460.
Qiu, J., et al. (2001) Cell cycle-dependent and DNA damage-inducible nuclear localization of FEN-1 nuclease is consistent with its dual functions in DNA replication and repair.J. Biol. Chem. 276:4901-4908.
Rumbaugh, J.A., et al. (1998) Processing of an HIV replication intermediate by the human DNA replication enzyme FEN1.J. Biol. Chem. 273:28740-5.
Sharma, P. L, and Crumpacker, C. S. (1999) Decreased processivity of human immunodeficiency virus type 1 reverse transcriptase (RT) containing didanosine-selected mutation Leu74Val: a comparative analysis of RT variants Leu74Val and lamivudine-selected Met184Val.J. Virol. 73:8448-856.
Shen, B., et al. (1996) Essential amino acids for substrate binding and catalysis of human flap endonuclease-1.J. Biol. Chem. 271:9173-9176.
Shen, B., et al. (1998) Flap endonuclease homologues in archaebacteria exist as independent proteins.TiBS. 23:171-173.
Shibata, Y., and Nakamura, T. (2002) Defective flap endonuclease 1 activity in mammalian cells is associated with impaired DNA repair and prolonged S phase delay.J. Biol. Chem. 277:746-754.
Stevenson, M. (2000) HIV nuclear import: What's the flap?Nat. Med. 6:626-8.
Whitwam, T., et al. (2001) Identification of a central DNA flap in feline immunodeficiency virus.J. Virol. 75:9407-14.
Yam, P. Y., et al. (2002) Design of HIV vectors for efficient gene delivery into human hematopoietic cells.Mol. Ther. 5:479-84.
Yoder, K.E., and Bushman, F.D. (2000) Repair of gaps in retroviral DNA integration intermediates.J. Virol. 74:11191-11200.
Zennou, V., et al. (2000) HIV-1 genome nuclear import is mediated by a central DNA flap.Cell101:173-85.
Zennou, V., et al. (2001) The HIV-1 DNA flap stimulates HIV vector-mediated cell transduction in the brain.Nat. Biotechnol. 19:446-50.
Zheng, L., et al. (2004) Novel function of the flap endonuclease-1 complex in processing stalled DNA replication forks.EMBO Reports6:83-89.
Shen Binghui
Zheng Li
City of Hope
Perkins Coie LLP
Sliger Lauren
Wang Shengjun
LandOfFree
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