Suppression of electroendosmosis during electrophoresis in gel-f

Details Suppression of electroendosmosis during electrophoresis in gel-f Suppression of electroendosmosis during electrophoresis in gel-f

1995-04-05

1996-08-13

Niebling, John

Chemistry: electrical and wave energy

Processes and products

Electrophoresis or electro-osmosis processes and electrolyte...

204451, 204601, G01N 2726, G01N 27447

Patent

active

055453023

ABSTRACT:
The phenomenon of electroendosmosis which occurs in electrophoretic separations and interferes with the efficiency, accuracy and reproducibility of the separations, is suppressed by the inclusion in the separation medium of a dissolved hydrophilic polymer which has been modified by amine groups covalently bonded thereto, in a controlled proportion relative to the polymer. This modified polymer suppresses electroendosmosis in both flee zone electrophoresis and molecular sieve electrophoresis. When this modified polymer is used in a capillary, the reduction which it produces in the electroendosmosis is the same as that achieved by coating the capillary wall with a neutral polymer.

REFERENCES:
patent: 5264101 (1993-11-01), Demorest et al.

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