Support matrices for immobilized enzymes

Details Support matrices for immobilized enzymes Support matrices for immobilized enzymes

1978-10-16

1980-06-03

Lesmes, George F.

Stock material or miscellaneous articles

Web or sheet containing structurally defined element or...

Composite having voids in a component

435180, B32B 326

Patent

active

042062594

ABSTRACT:
Organic-inorganic support matrices for immobilized enzymes comprise a solid, porous, inorganic, water insoluble support combined with a copolymeric material resulting from the reaction of aminopolystyrene and a bifunctional monomer. The matrix is prepared by depositing a salt of amino-polystyrene within the pores of the solid support from an aqueous solution at a pH less than 7 after which the resulting composite is then reacted with an excess of a bifunctional reactive monomer thus forming a copolymeric organic material in situ substantially entrapped in the pores of the support and containing functionalized pendent groups to which an enzyme may be coupled to form an immobilized enzyme conjugate.

REFERENCES:
patent: 3796634 (1974-03-01), Haynes et al.
patent: 3850751 (1974-11-01), Messing
patent: 4048018 (1977-09-01), Coughlin et al.
patent: 4066504 (1978-01-01), Krasnobajew et al.
Biotechnology and Bioengineering, "Enzyme Immobilization on Macroreticular Polystyrene", by George Baum, vol. XVII, pp. 253-270 (1975).

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