Support for the determination of enzyme activities and process

Details Support for the determination of enzyme activities and process Support for the determination of enzyme activities and process

1977-02-16

1981-07-07

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving glucose or galactose

435 4, 435 18, 435 19, 435 21, 435 23, 435 24, 435 29, 435301, 435805, 435287, 23230B, 422 56, 422 99, C12Q 154, C12Q 142, C12Q 100, C12M 120

Patent

active

042775612

ABSTRACT:
A support and method for the determination of enzyme activity in a biological extract is provided wherein the support comprises a fibrous material impregnated with a substrate and a water-soluble pH stabilizer comprising a first reagent suitable for a pH of enzyme activity over 7 or a second reagent suitable for a pH of enzyme activity of 7 or less; the viscose fibers are sufficiently loose in texture so as to be accessible both to products in solution and to tissuey and cellular mash or whole cells and sufficiently fine to permit adsorption and uniform distribution of reagents over the area thereof.
The method comprises:

REFERENCES:
patent: 3043669 (1962-07-01), Charles
patent: 3552925 (1971-01-01), Fetter
patent: 3616251 (1971-10-01), Linoli
patent: 3764479 (1973-10-01), Bergeron et al.
patent: 3842166 (1974-10-01), Bucalo
patent: 3843324 (1974-10-01), Edelman et al.
patent: 3963442 (1976-06-01), Bullard et al.
patent: 4038149 (1977-07-01), Liner et al.

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