Chemistry: molecular biology and microbiology – Plant cell or cell line – per se ; composition thereof;... – Culture – maintenance – or preservation techniques – per se
Patent
1996-12-20
1998-10-27
Scheiner, Toni R.
Chemistry: molecular biology and microbiology
Plant cell or cell line, per se ; composition thereof;...
Culture, maintenance, or preservation techniques, per se
435431, 47 44, 47 58, 47 485, A01G 3100, A01H 400, C12M 300
Patent
active
058277439
DESCRIPTION:
BRIEF SUMMARY
TECHNICAL FIELD
The present invention relates to a support (or carrier) which is suitable for the growth or regeneration of a plant. More specifically, the present invention relates to a method of promoting or accelerating the growth or regeneration of a plant while inhibiting the propagation of bacteria and fungi, by using a support for growing or regenerating the plant which is capable of effectively inhibiting the propagation of bacteria and fungi; and/or a support for growing or regenerating a plant which is capable of easily collecting or transferring (subculturing) the plant; and a method of growing or regenerating a plant by utilizing such a support.
BACKGROUND OF THE INVENTION
In recent years, it has attracted much attention to develop a technique for growing or regenerating a plant having a character suitable for an intended purpose.
Quite recently, on the basis of a technique for growing a plantlet from a growing (or vegetative) point, have been various e.g., those for regenerating a plant from an organ such as leaf, stem, root, petal, and anther (pollen) directly, or by way of a callus or protoplast. Particularly, at the stage of the protoplast, new breeding techniques such as the introduction of a foreign or exogeneous gene using a particle gun and electroporation have been developed, and there have been attempts to grown and regenerate a plant which is more suitable for an intended purpose.
In a case where these new breeding or plant tissue-culturing processes are put to practical use, it is the most important point, commonly in the above techniques, to establish a method of efficiently growing or regenerating a plant which has acquired the above-mentioned intended character.
In view of the physical property of a "culture medium" to be used for the culture process, the plant tissue-culturing processes are roughly classified into a method (liquid culture process) using a liquid culture medium, and a method (solid culture process) using a culture medium which has been converted into a gel state by using agar, gellangum (trade name: GELRITE, e.g., mfd. by San-ei Kagaku Kogyo K.K.), etc.
The liquid culture process in which a plant tissue or cells are grown in a suspension state, may facilitate the rapid and large-quantity multiplication thereof, and therefore such a method is suitable for a bioreactor, etc., which is to be used for the purpose of producing a secondary metabolite of a plant. However, in many cases, the liquid culture process is not suitable for the growth or regeneration of a plant which is intended for the multiplication of a clone. The reason for this is that, in the liquid culture process, a plant is grown in a state such that the plant is soaked in a culture medium, and therefore the resultant plant has an extremely poor resistance to dryness, and it is impossible to transfer the resultant plant into field (or farm) cultivation which is to be conducted at a relatively low humidity.
On the other hand, in the solid culture process using agar gel, etc., the gel state of a culture medium functions as a good support for a plant, and therefore such a method may solve the above-mentioned problem of "soaking in liquid" which will occur in the case of the liquid culture process. Accordingly, the solid culture process has widely been used for growing or regenerating a plant.
However, with respect to the current process (for growing or regenerating a plant (i.e., solid culture process) by using agar, etc., some serious problems as described below have been pointed out.
Thus, in the process for growing or regenerating a plant by using agar, it is extremely difficult to collect (or harvest) the resultant plant, which has been grown or regenerated in the gel-like support such as agar gel, in a state such that the plant is free from the agar gel. Particularly, when some roots are regenerated in the gel-like agar in the step of the formation of roots originating from a shoot, it is extremely difficult to separate such roots from the agar. The agar culture medium to be used for the root-for
REFERENCES:
patent: 5229288 (1993-07-01), Mori et al.
patent: 5273066 (1993-12-01), Graham et al.
Patterson, D. "Free Volume and Polymer Solubility: A Qualitative View." Macromolecules vol. 2, No. 6, Nov.-Dec. 1969: (672-677).
Tanaka, Toyoichi, et al. "Critical Kinetics of Volume Phase Transition of Gels" Physical Review Letters vol. 55, No. 22, 25 Nov. 1985: (2455-2458).
Obonai, Y. "Thermo-Responsive Hydro Gel (TRHG) For Tissue Culture of Orchids" Proceedings of NIOC'96 Nagoya 1996: (119-122).
Kawanishi, Kazuo et al. "The Sol-Gel Transition and the Liquid-Liquid Phase Separation in Poly(vinyl chloride) Solutions" Polymer Journal vol. 18, No. 5, 1986: (411-416).
Heskins, H., and J.E. Guillet. "Solution Properties of Poly(B-isopropylacrylamide)" J.Macromol.Sci.-Chem., A2(8), Dec., 1968: (1441-1455).
Mukoyama Orchids Ltd.
Scheiner Toni R.
LandOfFree
Support for growing/regenerating plant and method of growing/reg does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Support for growing/regenerating plant and method of growing/reg, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Support for growing/regenerating plant and method of growing/reg will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1613295