Support and method for immobilizing polypeptides

Details Support and method for immobilizing polypeptides Support and method for immobilizing polypeptides

1993-09-29

1997-02-11

Weimar, Elizabeth C.

Synthetic resins or natural rubbers -- part of the class 520 ser

Synthetic resins

Mixing of two or more solid polymers; mixing of solid...

436 89, 530334, A61K 3802

Patent

active

056022077

ABSTRACT:
A solid support and method for immobilization and sequence analysis of polypeptides. In one aspect, the invention is directed to a method for immobilizing a polypeptide on a solid support. In the method, a solid support having surface-attached carboxylic acid groups is reacted with an isoxazolium salt to form an activated support. After the activated support has been washed to remove residual isoxazolium salt and base, the support is dried. The dried support is then contacted with a polypeptide under conditions effective to bind the polypeptide covalently to the support. The immobilized polypeptide can be conveniently sequenced by N- and C-terminal sequencing methods.

REFERENCES:
patent: 4923901 (1990-05-01), Koester et al.
patent: 5041388 (1991-08-01), Boyd
patent: 5049507 (1991-09-01), Hawke et al.
patent: 5051368 (1991-09-01), Boyd
patent: 5185266 (1993-02-01), Boyd et al.
patent: 5304497 (1994-04-01), Boyd et al.
Boyd, V. L., et al., "Synthesis of the 2-Thiohydantoins of Amino Acids Using Woodward's Reagent K," Tetrahedron Letters 31(27): 3849-3852 (1990).
Pappin, D. J. C., et al., "Solid-Phase Sequence Analysis of Proteins Electroblotted or Spotted onto Polyvinylidene Difluoride Membranes," Analytical Biochemistry 187: 10-19 (1990).
Woodward, R. B., and R. A. Olofson, "The Reaction of Isoxazolium Salts with Nucleophiles," Tetrahedron Supplement 7: 415-440 (1966).

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