Supersecreting mutants of Saccharomyces cerevisiae

Details Supersecreting mutants of Saccharomyces cerevisiae Supersecreting mutants of Saccharomyces cerevisiae

1988-11-18

1991-10-15

Teskin, Robin L.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 694, 435 698, 435 699, 435212, 435219, 435226, 4353201, 4351723, 4351721, 435255, 435256, 435171, 935 47, 935 48, C12P 2100, C12N 116, C12N 118

Patent

active

050574167

ABSTRACT:
A screening procedure is provided which utilizes a milk clotting assay for selecting supersecreting yeast cells for obtaining high yields of desired polypeptide products.
Supersecreting yeast cells are provided as filed with American Type Culture Collection.
Final polypeptide products are obtained from mutant yeast strains which have been screened as to secreting properties with supersecreters then cultured to obtain high yields.

REFERENCES:
Sherman et al. Methods in Yeast Genetics pp. 3-8, 1982, "Isolation and Characterization of Auxotrophic, Temperature-Sensitive and UV-Sensitive Mutants".
Current Genetics, vol. 7, No. 1, 1983 Berlin, Heidelberg, N.Y., H. Bussey et al., "Protein Secretion in Yeast: Two Chromosomal Mutants that Oversecrete Killer Toxin in Sccharomyces cerevisiae" pp. 449-456, pp. 449, column 1, summary, p. 456, column 1, line 3-12.

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