Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Patent
1992-09-29
1995-01-10
Russel, Jeffrey E.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
435270, 435 911, 435 711, 435 712, 530423, 530426, 536 2541, 554 8, C07K 312, C07K 114, C12P 1934, C12P 2100
Patent
active
053808266
ABSTRACT:
The invention involves the supercritical or near-critical fluid disruption of microbial cells and extraction of intracellular components. First, a solvent that is a gas at ambient conditions and that has a critical temperature of between 0.degree. and 100.degree. C. is selected. This solvent is brought to near-critical pressure or higher and to near-critical temperature. The solvent then is combined with a slurry of cells to saturate the cells with the solvent under the prescribed conditions. Next, the pressure is released to cause a pressure drop which results in partial disruption of the cell membrane and release of solvent and other materials from the cell. Novel apparatus and associated methods are provided for carrying out the foregoing process continuously.
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Castor Trevor P.
Hong Glenn T.
Aphios Corporation
Gitomer Ralph
Russel Jeffrey E.
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