Superactive cellulase formulation using cellobiohydrolase-1...

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues

Reexamination Certificate

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Reexamination Certificate

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07449550

ABSTRACT:
Purified cellobiohydrolase I (glycosyl hydrolase family 7 (Cel7A) enzymes fromPenicillium funiculosumdemonstrate a high level of specific performance in comparison to other Cel7 family member enzymes when formulated with purified EIcd endoglucanase fromA. cellulolyticusand tested on pretreated corn stover. This result is true of the purified native enzyme, as well as recombinantly expressed enzyme, for example, that enzyme expressed in a non-nativeAspergillushost. In a specific example, the specific performance of the formulation using purified recombinant Cel7A fromPenicillium funiculosumexpressed inA. awamoriis increased by more than 200% when compared to a formulation using purified Cel7A fromTrichoderma reesei.

REFERENCES:
patent: 5536655 (1996-07-01), Thomas et al.
patent: 2002/0061560 (2002-05-01), Lawlis
patent: 2002/0155536 (2002-10-01), Van Den Brink et al.
Munoz et al. Family 7 cellobiohydrolases fromPhanerochaete chrysosporium: crystal structure of the catalytic module of Cel7D (CBH58) at 1.32 Å resolution and homology models of the isozymes, J Mol Biol. Dec. 14, 2001; Vo. 314 No. 5, pp. 1097-1111.
Ward, M. et al., WO/1992/006209, “Trichoderma Reesei Containing Deleted Cellulase and Other Enzyme Genes and Cellulase Combination Therefrom” Apr. 16, 1992. Abstract.
Brooks et al., Proc. Annu. Fuels Biomass Symp. 2nd.
Ghose et al., Biotechnol. Bioeng., (1984), 26 (4): pp. 377-381 (1984).
Spindler et al., Biotechnology Letters, 14:403-407 (1992).
Baker et al., Hydrolysis of Cellulose Using Ternary Mixtures of Purified Cellulases, Appl. Biochem. Biotechnol. 70/72, pp. 395-403 (1998).
Baker et al., “A Membrane-Reactor Saccharification Assay to Evaluate the Performance of Cellulases Under Simulated SSF Conditions,” Appl. Biochem. Biotechnol., 63/65 pp. 585-595 (1997).
Wu et al. Prog. Nucl. Acid. Res. Molec. Biol. 21:101-141 (1978).
Bhat, K. M. et al., “Characterization of the Major Endo-1,4-Beta-D-Glucanases from the Cellulase of Penicillium pinophilum/funiculosum” Biochem. Soc. Trans. 1989, vol. 17, No. 1, pp. 103-104.
Parr, S. R., “The Characterization of a Commercial Cellulase Product from Penicilium funiculosum by Fast Protein Liquid Chromatography,” 1985, vol. 13, No. 2, pp. 452-453.
Wood, T.M., et al., “The Isolation, Purification and Properties of the Cellobiohydrolase Component of Penicillium funiculosum Cellulase,” Biochem J. 1980, vol. 189, No. 1, pp. 51-65.

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