Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Patent
1991-06-19
1993-06-01
Hill, Jr., Robert J.
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
435 691, 4353201, 530350, 536 235, C07K 1300, C12N 1512, C12N 1563
Patent
active
052159167
ABSTRACT:
The present invention relates to novel glucocorticoid receptors that have greater affinity and specifity for glucocorticoid steroids than the naturally occurring receptors. More particularly, the invention relates to the altering of the equivalent of cysteine-656 of the rat glucocorticoid receptor to either serine or glycine for the production of super glucocorticoid receptors which retain full biological activity in intact cells and have higher affinity and specificity for glucocorticoid steroid binding than the original receptor. The invention further relates to the recombinant expression of such altered receptors in host cells.
REFERENCES:
Miesfeld et al., 1986, Genetic Complementation of a glucocorticaid receptor deficiency . . . cell vol. 46, 389-399.
Miller et al., 1988, Steroid binding to Hepatoma Tissue Culture Cell Placocorticoid Receptors . . . J. Biol. Chem. 263:15217.
Simons et al., 1987, Identification of Cysteine 656 as the amino acid of hepatoma . . . J. Biol. Chem. 262:9676.
Chakraborti et al., 1991, Creation of Super Glucocorticoid Receptors . . . J. Biol. Chem. 266(33):22075.
Chakraborti Pradip K.
Garabedian Michael J.
Simons, Jr. Samuel S.
Yamamoto Keith R.
Hill Jr. Robert J.
The United States of America as represented by the Department of
Ulm John D.
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