.sup.125 I and .sup.131 I labeled phospholipids

Details .sup.125 I and .sup.131 I labeled phospholipids .sup.125 I and .sup.131 I labeled phospholipids

1982-09-19

1986-02-18

Nucker, Christine M.

Drug, bio-affecting and body treating compositions

Radionuclide or intended radionuclide containing; adjuvant...

252319, 2523154, 252634, 424 9, A61K 4300, A61K 4900

Patent

active

045713324

ABSTRACT:
N-(3-(3-.sup.125 iodo, 4-hydroxybenzyl)propionyl)phosphatidylethanolamine[.sup.125 I-phenylpropionyl-PE], a nonexchangeable iodinated lipid of high specific radioactivity is disclosed, with a method for its synthesis as well as the synthesis of other iodinated lipids using a technique involving acylation of the amine nitrogen of a phospholipid, such as phosphatidylethanolamine, with an [.sup.125 I] or [.sup.131 I] substituted cyclic organic acid, for example, monoiodinated Bolton-Hunter reagent and purification by thin layer chromatography. The iodinated lipid may be quantitatively incorporated into vesicles for use as an accurate liposome tracer in in vitro and in vivo assays that require high levels of sensitivity unobtainable with most other lipid-labeling techniques.

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