Sunflower regeneration from cotyledons

Details Sunflower regeneration from cotyledons Sunflower regeneration from cotyledons

1989-12-14

1991-07-09

Weimar, Elizabeth C.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

43524049, 43524054, C12N 500

Patent

active

050305727

ABSTRACT:
A method for regeneration of sunflower plants via shoot induction from cotyledon explants prepared from non-germinated sunflower embryos is described. Several methods for preparing explants by dissection of mature and immature fully-formed embryos are provided. Explants are prepared to insure that substantially all of the induced shoots derive from single cotyledon cells. Appropriately prepared explants are cultured on a shoot induction medium containing a combination of BA and NAA. Induced shoots are rooted and the resulting plantlets are grown into mature sunflower plants. This regeneration method is efficient, rapid and employs a convenient explant source, sunflower seed. This regeneration method is particularly useful in combination with methods for introduction of foreign DNA into sunflower cells and tissue.

REFERENCES:
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Rogers et al. (1974) In Vitro 9:463-467.
Georgieva-Todorova et al. (1980) Proc. of the 9th Int. Sunflower Conf., Torremolinas, Spain, vol. 1:122-128.
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