Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide contains a tissue – organ – or cell...
Patent
1997-04-09
1999-09-28
Fox, David T.
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide contains a tissue, organ, or cell...
800281, 800286, 800306, 800312, 800314, 8003173, 8003201, 800322, 435 691, 435134, 4352523, 4353201, 435419, 435468, 536 236, 536 241, 536 245, A01H 500, A01H 510, C12N 1529, C12N 1582, C12P 764
Patent
active
059591757
ABSTRACT:
The present invention is directed to 5' regulatory regions of a sunflower albumin gene. The 5' regulatory regions, when operably linked to either the coding sequence of a heterologous gene or a sequence complementary to a native plant gene direct expression of the coding sequence or complementary sequence in a plant seed. The regulatory regions are useful in expression cassettes and expression vectors for the transformation of plants.
Also provided are methods of modulating the levels of a heterologous gene or native plant gene such as a fatty acid synthesis or lipid metabolism gene by transforming a plant with the subject expression cassettes and expression vectors.
REFERENCES:
patent: 5365015 (1994-11-01), Grierson et al.
patent: 5552306 (1996-09-01), Thomas et al.
patent: 5614393 (1997-03-01), Thomas et al.
Smith et al. Antisense RNA inhibition of polygalacturonase gene expression in transgenic tomatoes. Nature. 334:724-726, Aug. 1988.
Napoli et al. Introduction of a chimeric chalcone synthase gene into petunia results in reversible co-suppression of homologous genes in trans. The Plant Cell. 2:270-289, Apr. 1990.
Allen et al. Sequence and expression of a gene encoding an albumin storage protein in sunflower. Molecular General Genetics. 210:211-218, Dec. 1987.
Reddy et al. Isolation of a delta-6-desaturase gene from the cyanobacterium Synechocystis sp. strain PCC 6803 by gain-of-function expression in Anabaena sp. strain PCC 7120. Plant Molecular Biology. 27:293-300, May 1993.
Reddy et al. Expression of a Cyanobacterial delta-6-desaturase gene results in a gamma-linolenic acid production in transgenic plants. Nature Biotechnology. 14;639-642, May 1996.
Kim et al. A 20 nucleotide upstream element is essential for the nopaline synthase (nos) promoter activity. Plant Molecular Biology. 24;105-117, 1994.
Allen, et al. (1987) "Sequence and Expression of a Gene Encoding An Albumin Storage Protein in Sunflower", Mol. Gen. Genet., 210:211-218.
Beremand, P.D., et al. (1997) "Production of gamma-linolenic acid by transgenic plants expressing cyanobacterial or plant delta6-desaturase genes", Proc. Int. Symp. Plant Lipids, 12th: 351-353.
Topfer, et al. (1995) "Modification of Plant Lipid Synthesis", Science, 268:681-686.
Gibson, S., et al. (1994) "Cloning of a temperature-regulated gene encoding a chloroplast omega-3 desaturase from Arabidopsis thaliana", Plant Physiology, 104: 1615-1621.
Allen, et al. (1985) "Developmental Expression of Sunflower 11S Storage Protein Genes", Plant Molecular Biology, 5:165-173.
Brenner, R.R. (1976) "Regulatory Function of .DELTA.6 Desaturase--Key Enzyme of Polyunsaturated Fatty Acid Synthesis", Adv. Exp. Med. Biol., 83:85-101.
Cahoon, et al. (1992) "Expression of a Coriander Desaturase Results in Petroselinic Acid Production in Transgenic Tobacco", Proc. Natl. Acad. Sci USA, 89:11184-11188.
Crouch, et al. (1983) "cDNA Clones for Brassica napus Seed Storage Proteins: Evidence from Nucleotide Sequence Analysis that Both Subunits of Napin are Cleaved from a Precursor Polypeptide", J. Mol. Appl. Genet., 2:273-284.
Ericson, et al. (1986) "Structure of the Rapeseed 1.7 S Storage Protein, Napin, and Its Precursor", J. Biol. Chem., 261:14576-14581.
Higgins, et al. (1986) "Gene Structure, Protein Structure, and Regulation of the Synthesis of a Sulfur-rich Protein in Pea Seeds", J. Biol. Chem, 261:11124-11130.
Higgins, et al. (1987) "cDNA and Protein Sequence of a Major Pea Seed Albumin (PA 2:M.sub.r .about.26 000)", Plant Mol. Biol., 8:37-45.
Laroche-Raynal, et al. (1986) Identification and Characterization of the mRNA for Major Storage Proteins from Radish, Eur. J. Biochem., 157:321-327.
Ohlrogge, et al. (1991) "The Genetics of Plant Lipids", Biochim. et Biophys. Acta, 1082:1-26.
Ohlrogge, J.B. (1994) "Design of New Plant Products: Engineering of Fatty Acid Metabolism", Plant Physiol., 104:821-826.
Reddy, et al. (1996) "Expression of a Cyanobacterial .DELTA.6-Desaturase Gene Results in a .gamma.-Linolenic Acid Production in Transgenic Plants", Nature Biotech., 14:639-642.
Sun, et al. (1987) "Properties, Biosynthesis and Processing of a Sulfur-Rich Protein in a Brazil Nut (Bertholletia excelsa H.B.K.)", Eur. J. Bioch., 162:477-483.
Van de Loo, et al. (1995) "An Oleate 12-Hydroxylase from Ricinus communis L. is a Fatty Acyl Desaturase homolog", Proc. Natl. Acad. Sci USA, 92:6743-6747.
Weete, J.D. (1980) "Lipid Biochemistry of Fungi and Other Organisms", eds. Plenum Press, New York, pp. 59-62.
Youle et al. (1981) "Occurrence of Low Molecular Weight and High Cysteine Containing Albumin Storage Proteins in Oilseeds of Diverse Species", Amer. J. Bot., 68:44-48.
Beremand Phillip D.
Nunberg Andrew N.
Thomas Terry L.
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