Sulphocoumarin-containing nucleotides and their use in processes

Details Sulphocoumarin-containing nucleotides and their use in processes Sulphocoumarin-containing nucleotides and their use in processes

1994-01-21

1996-08-20

Jones, W. Gary

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 5, 435 6, 435 911, 536 243, 536 2433, 536 266, C12P 1934, C12Q 170, C12Q 168, C07H 2104

Patent

active

055478603

ABSTRACT:
The invention relates to the preparation, as substituents, of nucleotides possessing a fluorescent coumarin residue, the enzymic incorporation of these nucleotides into nucleic acids and the detection of nucleic acids of defined sequence by hybridization with a complementary, coumarin-labelled nucleic acid.

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Folsom et al., Anal. Biochem 182:309-314, 1989.
EP 0527433 A1, Novel Fluorescent label, Dattasupta et al pp. 1-10, 17 Feb. 1993, filing date Jun. 8, 1992.

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