Sulfonic acid group buffered dry analytical element for quantita

Details Sulfonic acid group buffered dry analytical element for quantita Sulfonic acid group buffered dry analytical element for quantita

1998-09-22

1999-10-26

Gitomer, Ralph

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

435 74, 435188, C12Q 150

Patent

active

059726350

ABSTRACT:
A dry analytical element for quantitatively analyzing creatine kinase is composed of a support and a creatine kinase-detecting agent layer. The creatine kinase-detecting agent layer contains creatine phosphate and adenosine diphosphate which react with each other to give adenosine triphosphate in the presence of creatine kinase, a buffer compound having a sulfonic acid group which keeps the layer in the range of pH 5.5 to pH 8.5 for the formation of adenosine triphosphate, and an indicator composition which reacts with the formed adenosine triphosphate to give a spectroscopically detectable compound. An analytical element for analysis of creatine kinase MB isozyme further contains in the layer an antibody which specifically inhibits activity of M sub-unit of creatine kinase.

REFERENCES:
patent: 4067775 (1978-01-01), Wurzburg et al.
patent: 4080265 (1978-03-01), Antonik
patent: 4387160 (1983-06-01), Gomez et al.
patent: 4912033 (1990-03-01), Ladenson et al.
patent: 5298406 (1994-03-01), Loyd et al.
patent: 5804394 (1998-09-01), Suzuki et al.
Landt Y. Semi-automated Direct Colorimetric Measurement of Creatine Kinase Isoenzyme MB Activity After Extraction From Serum by Use of a CK-MB Specific Monoclonal Antibody, Clin Chem 34/3 575-581, 1988.

Affiliated with

Also associated with

Rating

Sulfonic acid group buffered dry analytical element for quantita does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Sulfonic acid group buffered dry analytical element for quantita, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Sulfonic acid group buffered dry analytical element for quantita will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-762400