Sulfite reductase, the process for producing the same and...

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase

Reexamination Certificate

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Reexamination Certificate

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10453093

ABSTRACT:
The present invention relates to a purified sulfite reductase which has the following characteristics:a. It functions to catalyze the reduction of sulfites to sulfides or to recover the sulfhydryl groups from disulfide groups,b. In the aforesaid catalysis of the reduction, reduced nicotinamide dinucleotide phosphate (NADPH), methyl viologen (MVH) or other donors acts as an electron donor,c. Its molecular weight is from 100,000 to 400,000,d. The optimal temperature for its activity is from 20° C. to 30° C., ande. The optimal pH for its activity is from 6.5 to 8.0.The present invention also relates to a process for producing the purified sulfite reductase, and a method for recovering the proteins of denatured fish by using said sulfite reductase in solution or powder form.

REFERENCES:
patent: 6143556 (2000-11-01), Trachtenberg
Jiang et al. “Purified NADPH-sulfite reductase fromSaccharomyces cerevisiaeeffects on quality of ozonated Mackerel Surimi” Journal of Food Science 1998, 63: 777-781. entire document.
Journal of Food Science, A Publication of the Institute of Food Technologists, Nov./Dec. 2002, vol. 67, Nr. 9, 6 pages.
Purification and Characterization of Escherichia Coli Sulfite Reductase and its Application in Surimi Processing, Journal of Food Science, vol. 67, Nr. 9, 2002, pp. 3329-3334.

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