Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1986-03-28
1991-01-08
Rosen, Sam
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435 4, 435 25, 435176, C12Q 132
Patent
active
049835130
ABSTRACT:
A liquid coenzyme reagent comprises the coenzyme nicotinamide adenine dinucleotide (NAD), or nicotinamide adenine dinucleotide phosphate (NADP), and a sulfhydryl compound capable of counteracting the inhibitory effects of NAD (or NADP) degradation products on LD-L activity. The reagent is maintained at a pH of less than about 31/2. The liquid reagent has a shelf life of at least about 2 years, maintaining a LD-L activity of at least 90% that of the reagent when fresh, when stored at a temperature of from 2.degree.-8.degree. C.
REFERENCES:
patent: 3746625 (1973-07-01), Bergmeyer et al.
patent: 4067775 (1978-01-01), Wurzburg et al.
patent: 4229369 (1980-10-01), Green
patent: 4277562 (1981-07-01), Modrovich
patent: 4372874 (1983-02-01), Modrovich
Beckman Astra.sub.TM System LD-L Kit Brochure, 1984.
Beckman Liquid-Stat.sub.TM Liquid Enzyme Reagent Brochure, 1979.
Calbiochem-Behring Stat-Pack.sub.TM Ethyl Alcohol Test Brochure, 1983.
Kaplan et al., Effect of Alkali on Disphosphopyridine Nucleotide (1951), J. Biol. Chem., vol. 191, pp. 461-472.
Dalziel, The Purification of Nicotinamide Adenine Dinucleotide and the Kinetic Effects of Nucleotide Impurities (1963); J. Biol. Chem., vol. 238, pp. 1538-1543.
Dagaiczyk et al., The Effect of Cystein on L---Glycerophosphate and Lactate Dehydrogenase Reactions (1968), J. Biol. Chem., vol. 243, pp. 2236-2240.
Klotzch et al., Inhibitor-Contaminated NADH: Its Influence on Dehydrogenases and Dehydrogenase-Coupled Reactions (1969), Clin. Chem., vol. 15; p. 1056-1061.
Babson et al., Lactic Dehydrogenase Inhibitors in NAD (1970), Clin. Chem., vol. 16, 0. 254-255.
Guilbert et al., Investigation of the Open Ring Form of Nicotinamide Adenine Dinucleotide (1977), Biochemistry, vol. 16, pp. 355-344.
Margolis et al., Base-Catalyzed Rearrangement of NAD (1978), Fed. Proc., vol. 37, p. 1347.
Lin Tsung-I
Liu Alice C.
Beckman Instruments Inc.
Mak Danton K.
Rosen Sam
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