Sulfhydryl compounds for suppressing the inhibitory effects of N

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435 4, 435 25, 435176, C12Q 132

Patent

active

049835130

ABSTRACT:
A liquid coenzyme reagent comprises the coenzyme nicotinamide adenine dinucleotide (NAD), or nicotinamide adenine dinucleotide phosphate (NADP), and a sulfhydryl compound capable of counteracting the inhibitory effects of NAD (or NADP) degradation products on LD-L activity. The reagent is maintained at a pH of less than about 31/2. The liquid reagent has a shelf life of at least about 2 years, maintaining a LD-L activity of at least 90% that of the reagent when fresh, when stored at a temperature of from 2.degree.-8.degree. C.

REFERENCES:
patent: 3746625 (1973-07-01), Bergmeyer et al.
patent: 4067775 (1978-01-01), Wurzburg et al.
patent: 4229369 (1980-10-01), Green
patent: 4277562 (1981-07-01), Modrovich
patent: 4372874 (1983-02-01), Modrovich
Beckman Astra.sub.TM System LD-L Kit Brochure, 1984.
Beckman Liquid-Stat.sub.TM Liquid Enzyme Reagent Brochure, 1979.
Calbiochem-Behring Stat-Pack.sub.TM Ethyl Alcohol Test Brochure, 1983.
Kaplan et al., Effect of Alkali on Disphosphopyridine Nucleotide (1951), J. Biol. Chem., vol. 191, pp. 461-472.
Dalziel, The Purification of Nicotinamide Adenine Dinucleotide and the Kinetic Effects of Nucleotide Impurities (1963); J. Biol. Chem., vol. 238, pp. 1538-1543.
Dagaiczyk et al., The Effect of Cystein on L---Glycerophosphate and Lactate Dehydrogenase Reactions (1968), J. Biol. Chem., vol. 243, pp. 2236-2240.
Klotzch et al., Inhibitor-Contaminated NADH: Its Influence on Dehydrogenases and Dehydrogenase-Coupled Reactions (1969), Clin. Chem., vol. 15; p. 1056-1061.
Babson et al., Lactic Dehydrogenase Inhibitors in NAD (1970), Clin. Chem., vol. 16, 0. 254-255.
Guilbert et al., Investigation of the Open Ring Form of Nicotinamide Adenine Dinucleotide (1977), Biochemistry, vol. 16, pp. 355-344.
Margolis et al., Base-Catalyzed Rearrangement of NAD (1978), Fed. Proc., vol. 37, p. 1347.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Sulfhydryl compounds for suppressing the inhibitory effects of N does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Sulfhydryl compounds for suppressing the inhibitory effects of N, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Sulfhydryl compounds for suppressing the inhibitory effects of N will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-934218

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.