Suicide genes and new associations of pyrimidine nucleobase and

Details Suicide genes and new associations of pyrimidine nucleobase and Suicide genes and new associations of pyrimidine nucleobase and

1994-11-17

1999-01-05

Low, Christopher S. F.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

4353201, 4351721, 435325, 435353, 435354, 435363, 435366, 435 697, 435194, 435227, 514 44, 424 932, 935 14, 935 32, 935 62, 935 66, C12N 1585

Patent

active

058561536

ABSTRACT:
The present invention relates to two groups of suicide hybrid genes in which genes from one group specifically activate the pyrimidine nucleobase analog 5-fluorocytosine and genes from the other group activate the pyrimidine nucleoside analog azidothymidine to derivatives toxic for mammalian cells.The present invention further relates to methods for the selective killing of transfected tumor cells or immune cells using a single suicide hybrid gene or the combination of two suicide hybrid genes selected for a complementarity in their antimetabolic action. The present invention also relates to eukaryotic vectors comprising two expression suicide gene units, the first permitting the sensitization of tumor cells to 5-fluorocytosine and the second permitting the sensitization of HIV-infected cells to Azidothymidine in a synergistic fashion.

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