Sugarbeet storage-root-tissue-specific regulon

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide contains a tissue – organ – or cell...

Reexamination Certificate

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C435S320100, C435S410000, C435S419000, C435S468000, C536S023600, C536S024100, C800S298000

Reexamination Certificate

active

06248936

ABSTRACT:

The present invention relates to a new plant-based regulon that shows a storage root specific expression pattern.
Plants are often exposed to rapidly changing ambient conditions. This makes it advantageous for plants to be able to resist to quickly changing ambient influences. The adaption to new environment conditions is carried out via a change of expression in all kinds of genes. For example the Sh1-gene from maize is induced under anaerobic conditions. (Rowland et al., Mol. Gen. Genet. 218:33-40 (1989)). The situation for sucrose synthase genes from potato or rice is similar. (Salanoubat et al., Gene 84:181-185 (1989 ) and Wang et al. Plant Mol. Biol. 18:1191-1194 (1992)). A further environmental factor that contributes to an increased expression of sucrose synthase is a lower temperature. (Marana et al., Gene 88:167-172 (1990) and Crespi et al., Plant Physiol. 96:887-891 (1991)). Moreover, a limited supply of carbohydrates causes an increase in transcription of the maize gene Sh1, while under the same conditions the Susl gene is expressed with a reduced expression rate (Maas et al., EMBO J. 9:3447-3452 (1990) and Koch et al, Plant Cell 4:59-69 (1992)). Of wheat and arabidopsis it is moreover known that coldness treatment induces the sucrose synthase genes (Marana et al., Gene 88:167-172 (1990), Crespi et al., Plant Physiol. 96: 887-891 (1991) und Martin et al., Plant J. 4:367-377 (1993)). The maize gene Sh1, the potato's sucrose synthase gene and the wheat gene Ssl show an increased expression under anaerobic conditions (Rowland et al., Mol. Gen. Genet. 218:33-40 (1989), Salanoubat et al., Gene 84:181-185 (1989) and Marana et al., Gene 88:167-172 (1990)). Finally, the maize gene Sh1 is also influenced by an external supply of sucrose (Martin et al. Plant J. 4:367-377 (1993) and Koch et al., Plant Cell 4:59-69 (1992)).
The present invention addresses the technical problem of providing a new regulon which displays an expression pattern that is not yet known from any regulatory elements disclosed in the prior art and that can be advantageously used for the expression of genes under its control.


REFERENCES:
patent: 41 24 537 (1992-02-01), None
patent: WO94/28146 (1994-05-01), None
Guarente et al, Trends in Genetics, vol. 8, pp. 27-32, 1992.*
Huang et al, Plant Physiol., vol. 104, pp. 293-294, 1994.*
Rowland, L.S., Yen-Ching, C., and Chourey, P.S., (1989), “Anaerobic Treatment Alters the Cell Specific Expression of Adh-1, Sh, and Sus Genes in Roots of Maize Seedlings”,Mol. Gen. Genet., 218:33-40.
Fieuw, S. and Willenbrink, J. (1990), “Sugar Transport and Sugar-Metabolizing Enzymes in Sugar Beet Storage Roots (Beta vulgarisssp.altissima)”,J. Plant Physiol.,137:216-223.
Maraña, C., García-Olmedo, F., and Carbonero, P., (1990) “Differential Expression of Two Types of Sucrose Synthase-Encoding Genes in Wheat in Response to Anaerobiosis, Cold Shock and Light”Gene., 88:167-172.
Yang, N.S., and Russell, D., (1990), “Maize Sucrose Synthase-1 Promoter Directs Phloem Cell-Specific Expression of Gus Gene in Transgenic Tobacco Plants”,Proc. Natl. Acad. Sci. USA, 87:4144-4148.
Crespi, Martin D., Zabaleta, Eduardo J., Pontis, Horacio G., Salerno, Graciela L., (1991), “Sucrose Synthase Expression During Cold Acclimation in Wheat”,Plant Physiol.,96:887-891.
Martin, T., Frommer, W.B., Salanoubat, M., and Willmitzer, L., (1993), “Expression of an Arabidopsis Sucrose Synthase Gene Indicates a Role in Metabolization of Sucrose Both During Phloem Loading and in Sink Organs”,The Plant Journal, 4(2):367-377.
Sturm, A., {haeck over (S)}ebková, V., Lorenz, K., Hardeggar, M., Lienhard, S., and Unger, C., (1995) “Development-and Organ-Specific Expression of the Genes for Sucropse Synthase and Three Isoenzymes of Acid &bgr;-fructofuranosidase in Carrot”,Planta.195:601-610.
{haeck over (S)}ebková, V., Unger C., Hardeggar M., and Sturm A., (1995) “Biochemical, Physiological, and Molecular Characterization of Sucrose Synthase fromDaucus carota”,Plant Physiol.108:75-83.
Holger Hesse, Uwe Sonnewald and Lothar Willmitzer, (1995), Cloning and Expression Analysis of Sucrose-Phosphate Synthase from Sugar Beet (Beta vulgarisL.),Mol. Gen. Genet., 247:515-520.
Holger Hesse and Lothar Willmitzer, (1996), “Expression Analysis of a Sucrose Synthase Gene from Sugar Beet (Beta vulgarisL.)”,Plant Molecular Biology, 30:863-872.

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