Sugar chain synthesizer

Chemistry: molecular biology and microbiology – Apparatus – Including measuring or testing

Reexamination Certificate

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Details

C435S289100, C422S070000, C422S082050, C422S131000, C422S212000, C422S234000

Reexamination Certificate

active

06962809

ABSTRACT:
A sugar chain synthesizer comprising one or more reaction columns packed with immobilized glycosyltransferase and/or glycosidase; one or more separation means, arranged downstream from the reaction columns, for separating reaction products, unreaction products and byproducts contained in the solution eluted from the reaction columns; a first pump for feeding a primer of water soluble polymer and buffer solution to the reaction columns through a first selector valve; a second pump for feeding a buffer solution and sugar nucleotide solution to any one of the reaction columns through a second selector valve; one or more circulation flow paths connecting between a flow path downstream from the separation means and a flow path upstream from each of the reaction columns; and a third selector valve, arranged between the separation means and one or more circulation flow paths, for selective connection between the separation means and a desired circulation flow path. This synthesizer ensures continuous and automatic synthesis of sugar chains even if complicated.

REFERENCES:
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patent: 6329182 (2001-12-01), Pedersen et al.
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Gunter Jung et al., Staphyloferrin A: structurally new siderophore from staphylococci, Eur. J. Biochem. 191, 65-74 (1990).
Krista Witte et al., Enzymatic Glycoprotein Syntheses: Preparation of Ribonuclease Glycoforms via Enzymatic Glycopeptide Condensation and Glycosylation, J.Am. Chem. Soc. 1997, 119, 2114-2118.

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