Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part
Reissue Patent
1999-09-09
2004-02-24
Nelson, Amy J. (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Plant, seedling, plant seed, or plant part, per se
Higher plant, seedling, plant seed, or plant part
C435S320100, C435S411000, C435S419000, C435S468000, C536S023200, C536S023600, C800S284000
Reissue Patent
active
RE038446
ABSTRACT:
The present invention relates to the sucrose phosphate synthase (SPS), its process for preparation, its cDNA, and utilization of cDNA to modify the expression of SPS in the plant cells.
Difficulties in the purification of sucrose phosphate synthase (SPS) from plants have interferred with efforts to characterize this enzyme. SPS catalyses the formation of sucrose phosphate, the sucrose precursor molecule, from fructose-6 phosphate and UDP-glucose in photosynthetically active plant cells. Sucrose phosphatase then acts on the sucrose phosphate moiety, in an irreversible reaction, to remove the phosphate and to release sucrose ready to translocate from the mature leaf (source) to any tissue requiring photoassimilate (sink), especially growing tissues including young leaves, seeds, and roots.
Because SPS is considered a rate limiting enzyme in the pathway providing sucrose to growing tissue, the study of SPS and its activity is of special interest. In a recent publication, Walker, J. L. & Huber, S. C., Plant Phys. (1989) 89 : 518-524, the purification and preliminary characterization of spinach (Spinachia oleracea) SPS was reported. However, monoclonal antibodies specific to the spinach SPS were found to be non-reactive with all other plants tested, “closely related” and “relatively unrelated species”, including corn (Zea maize), soybean (Glycine max), barley (Hordeum vulgare), and sugar beet (Beta vulgaris). Thus, additional purified sources of SPS enzyme are needed for effective characterization of this factor. Especially of interest is the characterization of the corn SPS because of its very high export rates, as compared for example, to SPS levels of activity as found in the leaves of soybean.
With the advent of biotechnology, the ability to modify various properties of plants, especially agronomically important crops, is of interest. In this regard, it would be useful to determine the coding sequence for an SPS gene to probe other crop sources, to use such coding sequences to prepare DNA expression constructs capable of directing the expression of the SPS gene in a plant cell and to express a DNA sequence encoding an SPS enzyme in a plant to measure the effects on crop yield due to the increased rate of sucrose translocation to growing tissues.
REFERENCES:
patent: 5665892 (1997-09-01), Van Assche et al.
patent: 90402084.9 (1997-01-01), None
Sonnewald et al. Planta 189: 174-181, 1993.*
Kalt-Torres et al. Physiol. Plant. 70: 653-658,1987.*
Von Schaewen et al. EMBO J. 9: 3033-3044, 1993.*
Boswell et al. In Computational Molecular Biology Sources and Methods for Sequence Analysis (Lesk, ed.) Oxford University Press, Oxford, 1988, pp. 170-171.*
American Heritage Dictionary, second college edition, Houghton Mifflin Co., Boston, 1982, p. 1340.*
Kalt-Torres et al. (1987) Physiol, Plantarum 70: 653-658.*
Lee et al. (1988) Science 239: 1288-1291.*
Dickinson et al. (Feb. 1991) Plant Physiol. 95: 420-425.*
von Schaewen et al. (1990) The EMBO J. 9: 3033-3044.*
Sonnewald et al. (1993) Plant 189: 174-181.*
Werr et al. (1985) EMBO J. 4: 1373-1380.*
W. Kalt-Torres, et al., “Isolation and characterization of multiple forms of maize leaf sucrose-phosphate synthase,”Physiol. Plantarum(1987) 70:653-658.
Kerr, et al. “Resolution of two molecular forms of sucrose-phosphate synthase from maize, soybean and spinach leaves,”Planta(1987) 170:515-519.
Su, et al. “Purification and Properties of Sucrose Synthase from Maize Kernels,”Plant Physiology(1978) 61(3): 389-393.
Bruneau, et al. “Sucrose Phosphate Synthase, a Key Enzyme for Sucrose Biosynthesis in Plants,”Plant Physiol.(1991) 43-478.
Bruneau Jean Michel
Gervais Monica
Lando Danielle
Van Assche Charles
Voelker Toni Alois
Calgene, LLC.
Nelson Amy J.
Rae-Venter Barbara
Rae-Venter Law Group
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