Sucrose phosphate synthase

Details Sucrose phosphate synthase Sucrose phosphate synthase

2000-10-26

2001-11-27

Murthy, Ponnathapuachuta (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

C435S252300, C435S320100, C435S410000, C435S415000, C536S023200

Reexamination Certificate

active

06323015

ABSTRACT:

FIELD OF THE INVENTION
This invention is in the field of plant molecular biology. More specifically, this invention pertains to nucleic acid fragments encoding a sucrose biosynthetic enzyme in plants and seeds.
BACKGROUND OF THE INVENTION
In plants photosynthetically fixed carbon is ultimately converted into two main carbohydrate products, sucrose and starch. Sucrose is the form in which most fixed carbon is exported from the photosynthetic cell. Sucrose is then translocated to various parts of the plant which have a need for this sugar such as regions of active growth and developing seeds or tubers. Sucrose is synthesized in the cytoplasm of photosynthetic cells from the precursor dihydroxyacetone phosphate (DiHOAcP). In the last two steps of sucrose biosynthesis UDP-glucose is converted to sucrose by the successive action of sucrose phosphate synthase (SPS) (E.C. 2.4.1.14) and sucrose phosphatase. By modulating the level of SPS in plants it may be possible to control carbon partitioning in photosynthetic cells. Accordingly, the availability of nucleic acid sequences encoding all or a portion of a sucrose phosphate synthase protein would facilitate studies to better understand carbon partitioning in plants.
Worrell, A. C. et al. ((1991)
Plant Cell
3:1121-1130) describe a maize cDNA that encodes a sucrose phosphate synthase as confirmed by the ability of the cloned sequence to direct sucrose phosphate synthesis in
E. coli.
SUMMARY OF THE INVENTION
The instant invention relates to isolated nucleic acid fragments encoding a sucrose biosynthetic enzyme. Specifically, this invention concerns an isolated nucleic acid fragment encoding a sucrose phosphate synthase. In addition, this invention relates to a nucleic acid fragment that is complementary to the nucleic acid fragment encoding a sucrose phosphate synthase.
An additional embodiment of the instant invention pertains to a polypeptide encoding all or a substantial portion of a sucrose phosphate synthase.
In another embodiment, the instant invention relates to a chimeric gene encoding a sucrose phosphate synthase, or to a chimeric gene that comprises a nucleic acid fragment that is complementary to a nucleic acid fragment encoding a sucrose phosphate synthase, operably linked to suitable regulatory sequences, wherein expression of the chimeric gene results in production of levels of the encoded protein in a transformed host cell that is altered (i.e., increased or decreased) from the level produced in an untransformed host cell.
In a further embodiment, the instant invention concerns a transformed host cell comprising in its genome a chimeric gene encoding a sucrose phosphate synthase, operably linked to suitable regulatory sequences. Expression of the chimeric gene results in production of altered levels of the encoded protein in the transformed host cell. The transformed host cell can be of eukaryotic or prokaryotic origin, and include cells derived from higher plants and microorganisms. The invention also includes transformed plants that arise from transformed host cells of higher plants, and seeds derived from such transformed plants.
An additional embodiment of the instant invention concerns a method of altering the level of expression of a sucrose phosphate synthase in a transformed host cell comprising: a) transforming a host cell with a chimeric gene comprising a nucleic acid fragment encoding a sucrose phosphate synthase; and b) growing the transformed host cell under conditions that are suitable for expression of the chimeric gene wherein expression of the chimeric gene results in production of altered levels of a sucrose phosphate synthase in the transformed host cell.
An additional embodiment of the instant invention concerns a method for obtaining a nucleic acid fragment encoding all or a substantial portion of an amino acid sequence encoding a sucrose phosphate synthase.


REFERENCES:
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patent: 0 466 995 A2 (1992-01-01), None
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