Subunit respiratory syncytial virus vaccine preparation

Details Subunit respiratory syncytial virus vaccine preparation Subunit respiratory syncytial virus vaccine preparation

1996-07-12

2000-02-01

Mosher, Mary E.

Chemistry: molecular biology and microbiology

Virus or bacteriophage, except for viral vector or...

435239, 435236, 4242111, C12N 700, C12N 704, A61K 39155

Patent

active

060201827

ABSTRACT:
The fusion (F) protein, attachment (G) protein and matrix (M) protein of respiratory syncytial virus (RSV) are isolated and purified from respiratory syncytial virus by mild detergent extraction of the proteins from concentrated virus, loading the protein onto a hydroxyapatite or other ion-exchange matrix column and eluting the protein using mild salt treatment. The F, G and M proteins, formulated as immunogenic compositions, are safe and highly immunogenic and protect relevant animal models against respiratory syncytial virus.

REFERENCES:
Ewasyshyn, M.E. and Klein, M.H.; "Prospects for Immunization against Respiratory Syncytial Virus" Vaccine Research, vol. 2, 1993, pp. 263-272, XP002044888.
Walsh, E.E. et al: "Purification and Characterization of the Respiratory Syncytial Virus Fusion Protein" The Journal of General Virology, vol. 66, 1985, pp. 409-416 XP002044889.
Randolph et al. "Attenuated temperature sensitive respiratory syncitial virus mutant generated by cold adaptation". Virus Research, vol.33:241-259, 1994.
Murphy et al. "An update on approaches to the development of respiratory syncitial viral (RSV) and parainfluenza virus type 3 (PIV3) vaccines". Virus Research, vol. 32:13-36, 1994.
Wathen et al. "Characterization of a Novel Respiratory Syncitial Virus Chimeric FG Glycoprotein Expressed Using a Baculovirus Vector". J. Gen. Virology. vol.70:2625-2635, 1989.
Peretz et al. "Comparison of the ability of formalin-inactivated respiratory syncitial virus, immunopurified F, G and N proteins and cell lysate to enhance pulmonary changes in Balb/c mice". Vaccine, vol. 10, Issue 2, pp. 113-118, 1992.
Levine et al. "The Envelope Proteins from Purified Respiratory Syncitial Virus Protect Mice from Intranasal Virus Challenge". Proceedings of the Society for Experimental Biology and Medicine, vol.190, No.4:349-56, Apr. 1989.
Alansari et al. "Molecular cloning and sequence analysis of the phosphoprotein, nucleocapsid protein, matrix protein and 22K (M2) protein of the ovine respiratory syncitial virus". J. Gen. Virology, vol.75:3597-3601, 1994.

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