Subtilisin crystallization process

Details Subtilisin crystallization process Subtilisin crystallization process

1990-11-13

1991-08-20

Brown, Johnnie, R.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435187, 435188, 435219, 435221, 435272, 4352525, 435803, 435814, 435816, 435832, 435839, C12N 900, C12N 950, C12N 998, C12N 120

Patent

active

050413775

ABSTRACT:
Crystalline subtilisin is produced by adding a halide salt, such as sodium chloride or calcium chloride, to a concentrated subtilisin solution (at least about 40 g/1). This process does not produce amorphous subtilisin even at high salt concentrations in the solution. Optionally, subtilisin seed crystals also may be added to the concentrate to speed up the crystallization process.

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Bryan et al., Chemical Abstracts, 107: 171427b (1987) "Proteases of Enhanced Solubility: Characterization of Thermostable Variant of Subtilisin".
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Gilliland, et al., "Crystallization and Preliminary X-ray Defraction Studies of Subtilisin GX from bacillus SP. GX 6644", The Journal of Biological Chemistry, (1987) vol. 262, No. 9, pp. 4280-4283.
Chandrasekaran et al., "Purification of Subtilisin by Single Step Affinity Chromotography", Analytical Biochemistry, 150, pp. 141-144 (1985).

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