Subtilin variant of enhanced stability and activity

Details Subtilin variant of enhanced stability and activity Subtilin variant of enhanced stability and activity

1992-11-25

1996-05-14

Fleisher, Mindy

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

536 237, 43525231, 435 713, C12N 100, C12N 120, C12N 1511, C12N 1531

Patent

active

055166822

ABSTRACT:
A subtilin mutant substituting isoleucine for Glu.sub.4 of the native sequence (SEQ ID NO: 7) exhibits a 57-fold improvement in stability, resisting modification of the dehydroalanine residue at position 5. This stable mutant exhibits 3-4 times the specific activity, in suppression of bacterial spore outgrowth, of the native bacteriocin. A method for site-specific mutagenesis, as well as the resulting mutant gene, plasmid and transformant is similarly set forth.

REFERENCES:
Short Protocols in Molecular Biology (2nd Ed.) F. M. Ausubel et al. (ed.) published 1992 by John Wiley & Sons, New York; pp. 8-18-8-21.
Banerjee et al. (1988) J. Biol. Chem. vol. 263(9):9508-9514.
Buchman et al. (1988) J. Biol. Chem. vol. 263(31):16260-16266.
Liu et al. (1991) J. Bacteriol. vol. 173(22):7387-7390.
Hansen et al. (1991) Nisin & Novel Antibiotics, Escom Science Publishers, Leiden, Germany pp. 287-302.
Glover (1984) Gene Cloning: the mechanics of DNA manipulation Chapman and Hall Ltd; London, England pp. 36-47.
Stahl et al. (1984) J. Bacteriol. vol. 158(2):411-418.
Liu et al. (1992) J. Biol. Chem vol. 267(35):25078-25085.

Affiliated with

Also associated with

Rating

Subtilin variant of enhanced stability and activity does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Subtilin variant of enhanced stability and activity, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Subtilin variant of enhanced stability and activity will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1895165