Subtilin variant of enhanced stability and activity

Details Subtilin variant of enhanced stability and activity Subtilin variant of enhanced stability and activity

1994-03-30

1996-11-19

Vogel, Nancy T.

Chemistry: natural resins or derivatives; peptides or proteins;

Peptides of 3 to 100 amino acid residues

25 or more amino acid residues in defined sequence

435 691, 4351723, C07K 1432, C12P 2100, C12N 1500

Patent

active

055764207

ABSTRACT:
A subtilin mutant substituting isoleucine for Glu.sub.4 of the native sequence (SEQ ID NO:7) exhibits a 57-fold improvement in stability, resisting modification of the dehydroalanine residue at position 5. This stable mutant exhibits 3-4 times the specific activity, in suppression of bacterial spore outgrowth, of the native bacteriocin. A method for site-specific mutagenesis, as well as the resulting mutant gene, plasmid and transformant is similarly set forth.

REFERENCES:
Banerjee et al., J. Biol. Chem., 263(9):9508-9514 (1988).
Buchmann et al. J. Biol. Chem., 263(31):16260-16266 (1988).
Liu et al., J. Bartenol. 173(22):7387-7390 (1941).
Hansen et al., Noin & Novel Artibiotics, Escom Sci. Publ., Leidon, Germany pp. 287-302 (1991).
Glover et al., Gene Cloning: The mechanics of DNA Manipulation Chapman and Hill LTO, London, England pp. 36-47 (1984).

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