Subtilases

Details Subtilases Subtilases

2011-08-30

2011-08-30

Carlson, Karen (Department: 1656)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C435S069100, C435S252300, C435S252310, C435S320100, C536S023200, C510S300000

Reexamination Certificate

active

08008057

ABSTRACT:
The present invention relates to methods for producing variants of a parent JP170 subtilase and of a parent BPN′ subtilase and to JP170 and BPN′ variants having altered properties as compared to the parent JP170/BPN′ subtilase.

REFERENCES:
patent: 5397705 (1995-03-01), Zukowski et al.
patent: 5798234 (1998-08-01), Engel et al.
patent: 5891701 (1999-04-01), Sloma et al.
patent: 6376227 (2002-04-01), Takaiwa et al.
patent: 6803222 (2004-10-01), Hatada et al.
patent: 7294499 (2007-11-01), Svendsen et al.
patent: 204 342 (1992-02-01), None
patent: 1209233 (2002-05-01), None
patent: 4197182 (1992-07-01), None
patent: WO 88/01293 (1988-02-01), None
patent: WO 92/17577 (1992-10-01), None
patent: WO 96/09396 (1996-03-01), None
patent: WO 98/56927 (1998-12-01), None
patent: WO 00/26230 (2000-05-01), None
patent: WO 01/66712 (2001-09-01), None
patent: WO 2004/067737 (2004-08-01), None
Almog et al, “The 0.93 A° Crystal Structure of Sphericase: A Calcium-loaded Serine Protease fromBacillus sphaericus,” Journal of Molecular Biology, vol. 332, pp. 1071-1082 (2003).
Davail et al, “Cold Adaptation of Proteins,” The Journal of Biological Chemistry, vol. 269, No. 26, pp. 17448-17453 (1994).
Derwent record for JP 7-62152/JP95062152-B2, “Detergent Compsn. Contg. Alkali Protease—Produced byBacillusSpecies Y and an Alkali Builder with Specified Acid Dissociation Constant Range,” Lion Corp. Derwent Primary Accession No. 1988-164153 (Jul. 5, 1995).
Graycar et al, “EngineeredBacillus lentusSubtilisins Having Altered Flexibility,” Journal of Molecular Biology, vol. 292, pp. 97-109 (1999).
Gupta et al, “Bacterial Alkaline Proteases: Molecular Approaches and Industrial Applications,” Applied Microbial Biotechnology, vol. 59, pp. 15-32 (2002).
Mei et al, “Engineering Subtilisin YaB: Restriction of Substrate Specificity by the Substitution of Gly124 and Gly151 with Ala,” Protein Engineering, vol. 11, No. 2, pp. 109-117 (1998).
Narinx et al, “Subtilisin from Psychrophilic Antarctic Bacteria: Characterization and Site-directed Mutagenesis of Residues Possibly Involved in the Adaptation to Cold,” Protein Engineering, vol. 10, No. 11, pp. 1271-1279 (1997).
Saeki et al, “Novel Oxidatively Stable Subtilisin-like Serine Proteases from AlkaliphilicBacillusspp.: Enzymatic Properties, Sequences, and Evolutionary Relationships,” Biochemical and Biophysical Research Communications, vol. 279, pp. 313-319 (2000).
Siezen, “Homology Modelling of the Catalytic Domain of Human Furin: A Model for the Eukaryotic Subtilisin-like Proprotein Convertases,” European Journal of Biochemistry, vol. 222, pp. 255-266 (1994).
Siezen, “Modelling and Engineering of Enzyme/Substrate Interactions in Subtilisin-like Enzymes of Unknown 3-Dimensional Structure,” in Subtilisin Enzymes: Practical Protein Engineering, R. Bott and C. Betzel., Ed., Plenum Press New York, pp. 63-73 (1996).
Siezen et al, “Subtilases: The Superfamily of Subtilisin-like Serine Proteases,” Protein Science, vol. 6, pp. 501-523 (1997).
Strausberg et al, Biotechnology vol. 13, No. (7), pp. 669-673 (1995).
Wintrode et al, Journal Biology and Chemistry, vol. 275, No. 41, pp. 31635-31640 (2000).
Miyazaki et al, JMB, J. Mol. Biol., vol. 297, pp. 1015-1026 (2000).
Gallagher et al, Prot Struc Funct Genet. vol. 10, No. 2, pp. 205-213 (1993).

Affiliated with

Also associated with

Rating

Subtilases does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Subtilases, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Subtilases will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2735649