Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Reexamination Certificate
2010-09-10
2011-12-27
Rao, Manjunath (Department: 1656)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
C435S069100, C435S222000, C435S252300, C435S252310, C435S254110, C510S300000
Reexamination Certificate
active
08084241
ABSTRACT:
The present invention relates to methods for producing variants of a parent TY145 subtilase and of a parent BPN′ subtilase and to TY145 and BPN′ variants having altered properties as compared to the parent TY145/BPN′ subtilase.
REFERENCES:
patent: 5260207 (1993-11-01), Pantoliano et al.
patent: 5362414 (1994-11-01), Outtrup et al.
patent: 5470733 (1995-11-01), Bryan et al.
patent: 5567601 (1996-10-01), Bryan et al.
patent: 5707848 (1998-01-01), Bryan et al.
patent: 5891701 (1999-04-01), Sloma et al.
patent: 5972683 (1999-10-01), Tsai
patent: 6511371 (2003-01-01), Outtrup et al.
patent: 6541234 (2003-04-01), Bryan
patent: 6541235 (2003-04-01), Bryan
patent: 7294499 (2007-11-01), Svendsen et al.
patent: 7727756 (2010-06-01), Svendsen et al.
patent: WO 92/17577 (1992-10-01), None
patent: WO 02/29024 (2002-04-01), None
patent: WO 2004/083362 (2004-09-01), None
Toma, S., et al., 1991, “Grafting of a calcium-binding loop of thermolysin to aBacillus subtilisneutral protease”, Biochemistry, vol. 30, No. 1, pp. 97-107.
Almog et al., J. Mol. Biol., vol. 332, pp. 1071-1082 (2003).
S. Davail et al., Purification, Characterization, and Sequence of the Heat-Labile Subtilisin from the Antarctic PsychrophileBacillusTA41, The Journal of Biological Chemmistry, 1994, pp. 1748-1753, vol. 269, No. 26.
K. Miyazaki et al., Directed Evolution Study of Temperature Adaptation in a Psychrophilic Enzyme, J. Mol. Biol., 2000, pp. 1015-1026, vol. 297.
E. Narinx et al., Subtilisin from psychrophilic antarctic bacteria: characterization and site-directed mutagenesis of residues possibly involved in the adaptation to cold, Protein Engineering, 1997, pp. 1271-1279, vol. 10, No. 11.
Pantoliano et al., Biochemstry, vol. 28, pp. 7205-7213 (1989).
Rost et al., J. Mol. Biol., vol. 232, pp. 584-599 (1993).
Servant, P., et al., “Production of Crylla and CryllBa toxins inBacillus sphaericusConfers Toxicity towardsAedes aegyptiand resistant Cutex populations”, Applied and Environmental Microbiology, vol. 65, pp. 3021-3026, 1999.
Servant, P., et al., “Production of Crylla and CryllBa toxins inBacillus sphaericusConfers Toxicity towardsAedes aegyptiand resistant Cutex populations”, UniProt Accession No. Q9S3L6, May 1, 2000.
R. J. Siezen et al., The superfamily of sibtilisin-like serine proteases, Protein Science, 1997, pp. 501-523.
Wati, M.R., et al., “Toxin degrading protease ofBacillus sphaericus,4343 amino acid precursor protease”, UniProt Accession No. 054327, Jun. 1, 1998.
P.L. Wintrode et al., Cold Adaptation of a Mesophilic Subtilisin-like Protease by Laboratory Evolution, The Journala of Biological Chemistry, 2000, pp. 31635-31640, vol. 275, No. 41.
Wintrode et al., Biochimica et Biophysica Acta, vol. 1549, pp. 1-8 (2001).
Draborg Henriette
Svendsen Allan
McNamara Kristin J.
Moore William W
Novozymes A/S
Rao Manjunath
LandOfFree
Subtilases does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Subtilases, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Subtilases will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4315077