Substratum for cell culture and a method for culturing and isola

Details Substratum for cell culture and a method for culturing and isola Substratum for cell culture and a method for culturing and isola

1983-08-08

1985-12-17

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

435948, C12N 500, C12R 191

Patent

active

045593043

ABSTRACT:
A substratum for cell culture which comprises a chemically modified collagen rich in either positive or negative charges when under culture conditions. The substratum is prepared by modifying the amino groups or carboxyl groups of collagen. The chemically modified collagen enhances the adherence and proliferation of animal cells much more actively than unmodified collagen in the presence or absence of bovine fetus serum. The cultured animal cells can be detached efficiently from the chemically modified collagen. This allows for highly selective isolation and recovery of the cultured animal cells which can be accomplished without incurring any injury from the chemically modified collagen.

REFERENCES:
patent: 4064007 (1977-12-01), Choay et al.
patent: 4223984 (1980-09-01), Miyata et al.
Grinnell et al., Attachment & Spreading of BHK Cells to Collagen Substrata, 1978, Proc. Nat. Acad. Sciences 75, 4408-4412.
Leighton et al., "Collagen Coated Cellulose Sponge", Science, vol. 155, pp. 1259-1261, 1967.
Hirtenstein et al., "Microcarriers for Animal Cell Culture", Third General Mtg. of ESCAT, (S. Karger ed., 1980), pp. 109-116.
Kleinman et al., "Role of Collagenous Matrices in the Adhesion and Growth of Cells", The Journal of Cell Biology, vol. 88, pp. 473-485, Mar. 1981.

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