Substrates for determination of enzyme activity and intermediate

Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues – Tripeptides – e.g. – tripeptide thyroliberin – etc.

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530337, 435 23, C07K 508, C07K 106, C12Q 138

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active

051150999

ABSTRACT:
Novel compounds represented by the following formula: ##STR1## wherein A represents a specific amino acid residue are excellent as substrates for determination of enzyme activity such as trypsin, etc. The compounds can be synthesized from novel arginyl-3-tert-alkyloxycarbonyl-4-nitroanilides by a novel process comprising a selective deprotection step whereby the protecting group on the .alpha.-amine group of arginine is removed in the presence of a hydrochloric acid, acetic acid and dimethylformamide mixture, but a tert-alkyl protecting group on the --COOH group of the nitroanilide ring is not removed by this step.

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