Substrates and method for determining enzymes

Details Substrates and method for determining enzymes Substrates and method for determining enzymes

1981-06-15

1983-10-11

Phillips, Delbert R.

Chemistry of carbon compounds

Miscellaneous organic carbon compounds

C-metal

549288, C07C10352

Patent

active

044091400

ABSTRACT:
A method is disclosed for determining the presence of an enzyme in a biological fluid, which includes the steps of contacting the fluid with a synthetic chromogenic substrate, which is an amino acid derivative of 7-amino-4-trifluoromethylcoumarin; incubating the substrate-containing fluid to effect enzymatic hydrolysis; and fluorometrically determining the presence of the free 7-amino-4-trifluoromethylcoumarin chromophore in the hydrolyzate.

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Eastman Laser Products (1979).
Chemical Structure of Courmarins 17, 393-402 (1979).
J. Org. Chem. (1980) 45, 2283-2287.

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