Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1979-04-23
1981-10-13
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
435 24, 424 2, 424 7, C12Q 138, C12Q 136
Patent
active
042949238
ABSTRACT:
A method is disclosed for determining the presence of an enzyme in a biological fluid, which includes the steps of contacting the fluid with a synthetic chromogenic substrate, which is an amino acid derivative of 7-amino-4-trifluoromethylcoumarin; incubating the substrate-containing fluid to effect enzymatic hydrolysis; and fluorometrically determining the presence of the free 7-amino-4-trifluoromethylcoumarin chromophore in the hydrolyzate.
REFERENCES:
patent: 4056519 (1977-11-01), Bobbitt et al.
patent: 4137225 (1979-01-01), Enkenstam et al.
patent: 4147692 (1979-04-01), Nagatsu et al.
patent: 4155916 (1979-05-01), Smith et al.
patent: 4167449 (1979-09-01), Garginlo et al.
patent: 4229528 (1980-10-01), Smith et al.
Zimmerman, et al., "A New Fluorgenic Substrate for Chymetypsin", Anal. Biochem., vol. 70, No. 1 (1976), pp. 258-262.
Hammond, et al., "Search for Efficient, Near V. V. Lasing Dyes I, Substituent Effects on Bicyclic Dyes", Appl. Phys., vol. 8, No. 4 (1975), pp. 311-314.
Hammond et al., "Search for Efficient, Nerve V. V. Lasing Dyes, II Aza Substitution in Bicylic Dyes", Appl. Phys., vol. 8, No. 4 (1975), pp. 315-318.
Kosower Physical Organic Chemistry, John Wiley & Sons, Inc., New York (1968), pp. 38, 45, 47, 49.
Smith et al., "Direct Photometric or Flurometric Assay of Proteinases Using Substrates Containing 7-Amino-4-Trifluoromethylcourmarin", Thrombosis Res., vol. 17, (1980), pp. 393-402.
Hine, Structural Effects on Equilibria in Organic Chemistry, John Wiley & Sons, N. Y. (1975), pp. 44, 92, 161.
Bissell Eugene R.
Smith Robert E.
Barbeau Donald L.
Figg Edward A.
Wiseman Thomas G.
LandOfFree
Substrates and method for determining enzymes does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Substrates and method for determining enzymes, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Substrates and method for determining enzymes will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-931689