Substrate trapping protein tyrosine phosphatases

Details Substrate trapping protein tyrosine phosphatases Substrate trapping protein tyrosine phosphatases

1998-09-01

1999-09-14

Wax, Robert A.

Drug, bio-affecting and body treating compositions

Enzyme or coenzyme containing

Hydrolases

435196, A61K 3846, C12N 916

Patent

active

059519797

ABSTRACT:
Novel protein tyrosine phosphatases in which the invariant aspartate residue is replaced with an alanine residue and which bind to a tyrosine phosphorylated substrate and are catalytically attenuated are described. Also described are methods of identifying tyrosine phosphorylated proteins which complex with the described protein tyrosine phosphatases.

REFERENCES:
patent: 5352660 (1994-10-01), Pawson
patent: 5585233 (1996-12-01), Moller et al.
patent: 5589375 (1996-12-01), Ullrich et al.
Sun, et al., "MKP-1 (3CH134), an Immediate Early Gene Product, Is a Dual Specificity Phosphatase That Dephosphorylates MAP Kinase In Vivo", Cell 75:487-493 (1993).
Barford, et al., "Crystal Structure of Human Protein Tyrosine Phosphatase 1B", Science 263:1397-1404 (1994).
Songyang, et al., "SH2 Domains Recognize Specific Phosphopeptide Sequences", Cell 72:767-778 (1993).
Songyang, et al., "Catalytic specificity of protein-tyrosine kinases is critical for selective signalling", Nature 373:536-539 (1995).
Jia, et al., "Structural Basis for Phosphotyrosine Peptide Recognition by Protein Tyrosine Phosphatase 1B", Science 268:1754-1758 (1995).
Barford, et al., "Protein tyrosine phosphatases take off", Nature Struc. Biol. 2(12):1043-1053 (1995).
Zhang, et al., "Dissecting the catalytic mechanism of protein-tyrosine phosphatases", Proc. Natl. Acad. Sci. USA 91:1624-1627 (1994).
Tonks, "Introduction: protein tyrosine phosphatases", Seminars in Cell Biol. 4:373-377 (1993).
Cho, et al., "Substrate specificities of catalytic fragments of protein tyrosine phosphatases (HPTP.beta., LAR, and CD45) toward phosphotyrosylpeptide substrates and thiophosphotyrosylated peptides as inhibitors", Protein Sci. 2:977-984 (1993).
Dechert, et al., "Comparison of the specificity of bacterially expressed cytoplasmic protein-tyrosine phosphatases SHP and SH-PTP2 towards synthetic phosphopeptide substrates", Eur. J. Biochem. 231:673-681 (1995).
Ruzzene, et al., "Specificity of T-cell protein tyrosine phosphatase toward phosphorylated synthetic peptides", Eur. J. Biochem. 211:289-295 (1993).
Zhang, et al., "Protein Tyrosine Phosphatase Substrate Specificity: Size and Phosphotyrosine Positioning Requirements in Peptide Substrates", Biochemistry 33:2285-2290 (1994).
Garton and Tonks, "PTP-PEST: a protein tyrosine phosphatase regulated by serine phosphorylation", EMBO J. 13(16):3763-3771 (1994).
Flint, et al., "Multi-site phosphorylation of the protein tyrosine phosphatase, PTP1B: identification of cell cycle regulated and phorbol ester stimulated sites of phosphorylation", EMBO J. 12(5):1937-1946 (1993).
Kanner, et al., "The SH2 and SH3 domains of pp60.sup.src direct stable association with tyrosine phosphorylated proteins p130 and p110", EMBO J. 10(7):1689-1698 (1991).
Mayer and Hanafusa, "Mutagenic Analysis of the v-crk Oncogene: Requirement for SH2 and SH3 Domains and Correlation between Increased Cellular Phosphotyrosine and Transformation", J. Virol. 64:3581-3589 (1990).
Auvinen, et al., "Ornithine Decarboxylase-and ras-Induced Cell Transformations: Reversal by Protein Tyrosine Kinase Inhibitors and Role of pp130.sup.CAS ", Mol. Cell. Biol. 15(12):6513-6525 (1995).
Charbonneau and Tonks, "1002 Protein Phosphatases?", Annu. Rev. Cell Biol. 8:463-493 (1992).
Milarski, et al., "Sequence Specificity in Recognition of the Epidermal Growth Factor Receptor by Protein Tyrosine Phosphatase 1B", J. Biol. Chem. 268(31):23634-23639 (1993).
Eckstein, Jens W. et al., "Identification of an Essential Acidic Residue in Cdc25 Protein Phosphatase and a General Three-Dimensional Model for a Core Region in Protein Phosphatases", Protein Science, 5:5-12 (1996).
Yang, Qing et al., "Cloning and Expression of PTP-PEST", The Journal of Biological Chemistry, 268(9):6622-6628 (1993).
Yang, Qing et al., "Cloning and Expression of PTP-PEST, a Novel Human Nontransmembrane Protein Tyrosine Phosphatase", The Journal of Biological Chemistry, 268(23):17650 (1993).
Liu, Feng et al., "Direct Binding of the Proline-rich Region of Protein Tyrosine Phosphatase 1B to the Src Homology 3 Domain of p130.sup.Cas ", The Journal of Biological Chemistry, 271(49):31290-31295 (1996).
Brautigan, David L. and Pinault, Fran M., "Serine Phosphorylation of Protein Tyrosine Phosphatase (PTP1B) in HeLa Cells in Response to Analogues of cAMP or Diacylglycerol Plus Okadaic Acid", Molecular and Cellular Biochemistry 127/128:121-129 (1993).
Flint, Andrew J. et al., "Development of "Substrate-Trapaping" Mutants to Identify Physiological Substrates of Protein Tyrosine Phosphatases", Proc. Natl. Acad. Sci. U.S.A., 94:1680-1685 (1997).
Garton, Andrew J. et al., "Identification of p130.sup.cas as a Substrate for the Cytosolic Protein Tyrosine Phosphatase PTP-PEST", Molecular and Cellular Biology, 16(11):6408-6418 (1996).
Seely, B. Lynn et al., "Protein Tyrosine Phosphatase 1B Interacts With the Activated Insulin Receptor", Diabetes, 45(10):1379-1385 (1996).
Wu, et al., "Probing the Function of Asp128 in the Low Molecular Weight Protein-tyrosine Phosphatase Catalyzed Reaction. A Pre-steady-state and Steady-state Kinetic Investigation", Biochemistry, 35:5426-5434 (1996).
Eckstein et al., "Identification of an Essential Acidic Residue in Cdc25 Protein Phosphatase and a General Three-dimensional Model for a Core Region in Protein Phosphatases", Protein Science, 5(1):5-12 (1996).
Segel, Iriwn H., "Biochemical Calculations: How to Solve Mathematical Problems in General Biochemistry", Second Edition, John Wiley and Sons, New York, NY, pp. 277-279 (1976).
Zhang, Zhong-Yin, et al., "Expression, Purification, and Physicochemical Characterization of a Recombinant Yersinia Protein Tyrosine Phosphatase", J. Biol. Chem. 267(33): 23759-23766 (1992).
Denu, John M., et al., "The Catalytic Role of Aspartic Acid-92 in a Human Dual-Specific Protein-Tyrosine-Phosphatases", Biochemistry, 34:3396-3403 (1995).
Zhang, Zhongtao, et al., "Asp.sup.129 of the Low Molecular Weight Protein Tyrosine Phosphatase Is Involved in Leaving Group Protonation", J. Biol. Chem., 269(42):25947-25950 (1994).
Denu, John M., et al., "Visualization of Intermediate and Transition-State Structures in Protein-Tyrosine Phosphatase Catalysis", Proc. Natl. Acad. Sci. USA, 93:2493-2498 (1996).

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