Substrate solution for carboxylic ester hydrolase determination

Details Substrate solution for carboxylic ester hydrolase determination Substrate solution for carboxylic ester hydrolase determination

1975-09-22

1977-05-10

Monacell, A. Louis

Textiles: fiber preparation

195 99, G01N 3114

Patent

active

040226670

ABSTRACT:
A clear, stable aqueous reagent solution comprising a mixture of a water insoluble vinyl ester of an alkyl, alkenyl, or alkyl-aryl carboxylic acid, nonionic detergents, inorganic salts, enzyme activators, and a buffer, which when combined with a suitable acetaldehyde detection system is useful for the analysis of the action of certain carboxylic ester hydrolases.

REFERENCES:
gomori "Human Esterases" J. Lab. & Clin. Med. (1953) vol. 42, pp. 445-453.
Wills "The Effect of Surface-Active Agents on Pancreatic Lipase," Bioch. 60 (1955) pp. 529-534.
Kaplan et al. "Interaction of Beef Liver Lipase With Mixed Micellas of Tripalmitin and Triton X-100," J. Lipid Research, 12 (1971) pp. 321-330.
Brockerhoff "Substrate Specificity of Pancreatic Lipase" Biochimica et Biophysica Acta 212 (1970) pp. 92-101.
Bergmeyer "Methods of Enzymatic Analysis" 2nd Printing, Revised, Academic Press, New York & London, pp. 290-292.
Whitaker "A Rapid and Specific Method for the Determination of Pancreatic Lipase in Serum and Urine" Clinica Chimica Acta, 44 (1973) pp. 133-138.

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