Substrate solution for carboxylic ester hydrolase determination

Details Substrate solution for carboxylic ester hydrolase determination Substrate solution for carboxylic ester hydrolase determination

1977-11-22

1980-07-15

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 26, 252408, G01N 3114, G01N 3122

Patent

active

042129397

ABSTRACT:
A clear, stable aqueous reagent solution comprising a mixture of a water insoluble vinyl ester of an alkyl, alkenyl, or alkyl-aryl carboxylic acid, nonionic detergents, inorganic salts, enzyme activators, and a buffer, which when combined with a suitable acetaldehyde detection system is useful for the analysis of the action of certain carboxylic ester hydrolases.

REFERENCES:
patent: 4022667 (1977-05-01), Myrick et al.
The Merck Index, 8th Ed., Stecher (Ed)., Merck & Co., Inc., 1968 Rahway, N.J., p. 360.
Brockerhoff, Biochim. Biophys. Acta, 212, (1970), pp. 92-101.
Whitaker, Clinica Chimica Acta, 44 (1973), pp. 133-138.
Gomori, J. Lab. & Clin. Med., 42 (1953), pp. 445-453.

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