Substrate for the determination of desoxy-ribonuclease

Details Substrate for the determination of desoxy-ribonuclease Substrate for the determination of desoxy-ribonuclease

1974-09-03

1976-11-09

Naff, David M.

Textiles: fiber preparation

195 99, 1951035R, 424 2, 424 12, G01N 3300, C12K 104

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active

039909468

ABSTRACT:
A desoxy-ribonuclease substrate is prepared by boiling and homogenizing an aqueous dispersion of high molecular weight desoxy-ribonucleic acid (DNA) and 2-amino-7-dimethylamino-3-methyl diphenazthionium chloride (toluidine blue O) until a clear blue-colored solution is obtained containing a complex compound of the DNA and toluidine blue O. The substrate is used in tests for determination of desoxy-ribonuclease and it's antibodies.

REFERENCES:
patent: 3042587 (1962-07-01), Baumgarten et al.
Tiesler "Diagnosis and Evaluation of Antistreptodornase B" Saarlaendisehes Aerzteblatt, Mar. 1973.
Nelson et al., J. Lab. and Clin. Med. 71, 867-873, (May, 1968).
Streitfeld et al., J. Bacteriol. 84, 77-80 (1962).
Ruth F. Itzhaki "Binding of Polylysine and Toluidine Blue to Deoxy-ribonucleoprotein" Biochem. J. 121 (1) 25p-26p, 1971.

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