Substrate for PP2C

Details Substrate for PP2C Substrate for PP2C

2005-08-23

2005-08-23

Wax, Robert A. (Department: 1651)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

C435S196000, C435S007100, C514S012200, C514S075000, C530S352000

Reexamination Certificate

active

06933127

ABSTRACT:
The invention relates to a combination of a protein phosphatase type 2C as an enzyme and a phospho-BAD as substrate. The protein BAD is dephosphorylated in position Ser155. Furthermore, the combination is used in an in vitro screening for ligands which modulate protein phosphatase type 2C, comprising the steps: incubating the protein phosphatase type 2C in combination with the substrate phospho-BAD and the ligand of the assay and detecting the decrease in phospho-BAD and/or the increase in phosphate and/or BAD. Therefore, new drugs for the treatment apoptosis may be found.

REFERENCES:
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patent: WO 99/02167 (1999-01-01), None
patent: WO 99/27134 (1999-06-01), None
patent: WO 00/35946 (2000-06-01), None
patent: WO 01/10888 (2001-02-01), None
patent: WO 02/02629 (2002-01-01), None
patent: WO 02/13798 (2002-02-01), None
patent: WO 02/064737 (2002-08-01), None
Klumpp et al., “Purification and characterization of protein phosphatase type 2C in photoreceptors,” Methods in Enzymology 315:570-578-, 2000.
Wang et al., “Ca2+-induced apoptosis through calcineurin dephosphorylation of BAD,” Science 284:339-343, 1999.
Abstract of EP 0 566 535 A1—English.
XP-001098159—Review Article—Serine/threonine protein phosphatases, Stefaan WERA et al., Biochem J. (1995) 311, 17-29 (Printed in Great Britain) pp. 39-51.
XP008022476—Syndromes of Accelerated Atherosclerosis: Role of Vascular Injury And Smooth Muscle Cell Proliferation, John H. IP et al., JACC vol. 15, No. 7, 1990 by the American College of Cardiology, pp. 1667-1687.
XP-002211494—Protein phosphatase 2A activates the proapoptotic function of BAD in interleukin-3-dependent lymphoid cells by a mechanism requiring 14-3-3 dissociation, Chi-Wu Chiang et al., BLOOD, Mar. 1, 2001—vol. 97, No. 5, pp. 1289-1297.
U.S. Appl. No. 10/189,188.

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